Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

AMPIPROBE® Bacterial Vaginosis Assay Kit

Sensitive, specific and semi-quantitative qPCR assay for the detection of Bacterial Vaginosis
 
ENZ-GEN205-0100 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 
  • Sensitive, specific and semi-quantitative assay 
  • Low-cost alternative to other methods of Bacterial Vaginosis detection
  • Compatible with most open qPCR platforms
  • Smaller sample input that allows remaining extracted samples to be used in other tests
The AMPIPROBE® Bacterial Vaginosis (BV) Assay is a real-time PCR assay for the detection of Atopobium vaginae, Gardnerella vaginalis, Lactobacillus spp., Megasphaera spp., and BVAB2. The nucleotide sequences of the PCR primers have been optimized to target sequences within species-specific regions of the target genome. The BV primers have been designed using AMPIPROBE® technology which incorporates reporter and quencher dyes into the primers. Target detection is based on fluorescence decay when successive rounds of amplification bring fluorophore and quencher-labeled primer pairs in close proximity resulting in FRET. Loss of fluorescence below a defined threshold in a particular channel indicates sample positivity for the corresponding BV species.
The AMPIPROBE® BV Primer Mixes include primers for a ubiquitously conserved human housekeeping gene (human β-globin). Successful amplification of the internal control serves as an indicator of sample adequacy, extraction efficiency and successful amplification in each individual sample.
The AMPIPROBE® Bacterial Vaginosis Assay is a two tube assay and contains two separate BV primer mixes. Both BV primer mixes must be run with each sample in order to determine the positive or negative status of the sample.

Product Specification

Sensitivity:In a validation study approved by the New York State Department of Health, the AMPIPROBE® Bacterial Vaginosis Assay was determined to have a sensitivity of 84.30%.
 
Applications:qPCR
 
Application Notes:The AMPIPROBE® Bacterial Vaginosis Assay is compatible with any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN Rotor-Gene Q
 
Species reactivity:Atopobium vaginae, Gardnerella vaginalis, Lactobacillus spp., Megasphaera-1, BVAB-2
 
Specificity:In a validation study approved by the New York State Department of Health, the AMPIPROBE® Bacterial Vaginosis Assay was determined to have a specificity of 97.80%
 
Use/Stability:All components are stable at -20°C until the kit's expiration date.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Contents:AMPIGENE® HS Taq DNA Polymerase
AMPIGENE® dNTP Mix
AMPIPROBE® 5X Assay Buffer
AMPIPROBE® BV I Primer Mix
AMPIPROBE® BV II Primer Mix
BV Control 1
BV Control 2
BV Control 3
No Template Control (NTC)
Nuclease-free Water
 
Scientific Background:Bacterial vaginosis (BV) is the most common vaginal infection in the United States and affects approximately 29% of women. BV complications include an increased risk to develop other sexually transmitted infections and an increased risk of infection after pelvic surgery. In addition, BV during pregnancy has been linked with adverse pregnancy outcomes including preterm labor and delivery, low birth weight, premature rupture of membranes, postpartum metritis and intra-amniotic infection.
Clinical signs and symptoms (Amsel criteria) are often used to diagnose BV but the gold standard method for diagnosis is enumeration of bacterial morphotypes on a Gram-stained smear.
Although the exact etiology of BV is still undetermined, the consensus is that it occurs when Lactobacillus spp., which normally comprising 90-95% of total bacteria in healthy vaginal flora, is replaced by anaerobes, mainly but not restricted to Gardnerella vaginalis. More recently, molecular detection of other microbes, that are mostly uncultureable, have been associated with BV including Atopobium vaginae, BVAB2, and Megasphaera spp.
 

General Literature References

The microbiota of the vagina and its influence on women’s health and disease: D.H. Martin; Am. J. Med. Sci. 343, 2 (2012), Abstract; Full Text
Targeted PCR for Detection of Vaginal Bacteria Associated with Bacterial Vaginosis: D.N. Fredricks, et al.; J. Clin. Microbiol. 45, 3270 (2007), Abstract; Full Text
Vulvovaginal candidosis: J.D. Sobel; Lancet 369, 1961 (2007), Abstract;
Molecular identification of bacteria associated with bacterial vaginosis: D.N. Fredricks, et al.; N. Engl. J. Med. 353, 1899 (2005), Abstract; Full Text
Evaluation of vaginal complaints: M.R. Anderson, et al.; JAMA 291, 1368 (2004), Abstract;
Bacterial vaginosis in early pregnancy and pregnancy outcome: T. Kurki, et al.; Obstet. Gynecol. 80, 173 (1992 ), Abstract;
Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation: R.P. Nugent, et al.; J. Clin. Microbiol. 29, 297 (1991), Abstract;
Independent associations of bacterial vaginosis and Chlamydia trachomatis infection with adverse pregnancy outcome: M.G. Gravett, et al.; JAMA 256, 1899 (1986), Abstract;
Diagnosis of bacterial vaginosis by direct gram stain of vaginal fluid: C.A. Spiegel, et al.; J. Clin. Microbiol. 18, 170 (1983), Abstract;
Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations: R. Amsel, et al.; Am. J. Med. 74, 14 (1983), Abstract;

Related Products

AMPIGENE® qPCR 1-Step Probe Kit No-ROX 

Optimized  and efficient cDNA synthesis and probe-based qPCR in a single tube
PCR | Print as PDF
 
ENZ-NUC112-0200 200 Reactions 255.00 USD
 
ENZ-NUC112-1000 1000 Reactions 1,020.00 USD
Do you need bulk/larger quantities?
 

AMPIPROBE® HCV assay kit 

Sensitive and unique qPCR assay for quantifying HCV viral load
qPCR | Print as PDF
 
ENZ-GEN200-0100 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 

AMPIPROBE® Candida 2 Assay Kit 

Sensitive and specific qPCR assay for the detection of Candida species
qPCR | Print as PDF
 
ENZ-GEN203-0100 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 

AMPIPROBE® Candida 1 Assay Kit 

Sensitive and specific qPCR assay for the detection of Candida species
qPCR | Print as PDF
 
ENZ-GEN202-0100 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 

AMPIPROBE® NG/CT/TV Assay Kit 

Sensitive and specific qPCR assay for the detection of NG/CT/TV
qPCR | Print as PDF
 
ENZ-GEN207-0100 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 

AMPIPROBE® Ureaplasma spp. / M. genitalium / M. hominis Assay Kit 

Sensitive and specific qPCR assay for the detection of Ureaplasma spp. / M. genitalium / M. hominis
qPCR | Print as PDF
 
ENZ-GEN209-0100 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 

Related Literature

Brochures
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

Brochures
PCR & qPCR Complete PCR Solutions
PCR & qPCR Complete PCR Solutions
Download as PDF

Product Flyers
AMPIPROBE® HCV Assay Kit
AMPIPROBE® HCV Assay Kit
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2018 Enzo Life Sciences, Inc.,