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AMPIPROBE® Candida Extraction Controls

The AMPIPROBE® Candida Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Candida albicans, Candida Glabrata, Candida krusei, Candida parapsilosis and Candida tropicalis.
 
ENZ-GEN204-0500 500 µl Inquire for pricing
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  • Compatible with manual and automated DNA extraction methods
  • Compatible with most open qPCR platforms
The AMPIPROBE® Candida Extraction Controls is compatible with manual and automated DNA extraction methods as well as any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN QIAsymphony SP and Rotor-Gene Q.
All Performance specifications must be validated by end-user and will vary based on chosen extraction methods. Eluent from extraction can be used as extraction control for AMPIPROBE® Candida 1 Assay kit (Prod. No. ENZ-GEN202) and AMPIPROBE® Candida 2 Assay kit (Prod. No. ENZ-GEN203).
Plasmids in Extraction Controls are present at higher concentrations compared to assay controls included in the qPCR kit. Extra care should be taken to avoid contamination.

Product Specification

Applications:qPCR
 
Application Notes:The AMPIPROBE® Candida Extraction Controls is compatible with manual and automated DNA extraction methods as well as any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN QIAsymphony SP and Rotor-Gene Q.
 
Species reactivity:Candida albicans, Candida glabrata, Candida krusei, Candida parapsilosis, Candida tropicalis
 
Use/Stability:All components are stable at -20°C until the kit's expiration date.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Contents:Negative Extraction control
Candida Positive Extraction Control
 
Scientific Background:Vulvovaginal candidiasis (VVC) is second, after bacterial vaginosis, among the many causes of vaginitis, and is diagnosed in up to 40% of women with vaginal complaints in the primary care setting. Approximately 70-75% of women will have at least one symptomatic Candida vulvovaginal infection during their lives. Accurate detection and differentiation of Candida species that cause VVC leads to a significant improvement in clinical management of women with vaginal complaints, especially in women with recurrent VVC (RVVC).
The current assay provides rapid and accurate results for the detection of C. albicans, C. glabrata, and C. krusei DNA in a user-supplied sample of interest.
 
Protocol:SETUP of DNA Extraction control
The procedure described below is optimized for the QIAsymphony SP instrument (Qiagen) starting from an AMPTIMA® Multitest Swab Specimen collection tube.
Once thawed at room temperature, the AMPIPROBE Extraction Control tubes may be prepared. Label two 2.0 mL sample tubes with the appropriate control type (PC, NC), and then add 1.0 mL of blank APTIMA media to each tube.
Next, add 25 µL of each AMPIPROBE Extraction Control into their associated, labelled 2.0 mL tube. The extraction controls are now prepared and ready for use. Continue extraction procedure same as patient samples.

PCR SETUP
Please refer to the PCR SETUP protocol present in Product Manual AMPIPROBE® Candida 1 or Candida 2 Assay Kits (Catalog #: ENZ-GEN202-0100, 100 Assays or ENZ-GEN203-0100, 100 Assays) and treat the Candida Positive Extraction Control and Negative Extraction controls as Samples.
 
Note: The reagents required for the PCR Master Mix provided in the AMPIROBE® Candida 1 or Candida 2 Assay kits will be sufficient to run also the Candida Extraction Controls.
 
 

General Literature References

Vulvovaginal candidosis: J.D. Sobel; Lancet 369, 1961 (2007), Abstract;
Evaluation of vaginal complaints: M.R. Anderson, et al.; JAMA 291, 1368 (2004), Abstract;

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