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AMPIPROBE® Candida 2 Assay Kit

Sensitive and specific qPCR assay for the detection of Candida species
 
ENZ-GEN203-0100 100 tests Inquire for pricing
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  • Highly sensitive and specific assay
  • Low-cost alternative to other methods of Candida detection
  • Compatible with most open qPCR platforms
  • Smaller sample input that allows remaining extracted samples to be used in other tests
The AMPIPROBE Candida 2 Assay is a qualitative real-time PCR assay for the detection of C. parapsilosis and C. tropicalis genomic DNA. The nucleotide sequences of the PCR primers have been optimized to target sequences within species-specific regions of the Candida genome. The Candida primers have been designed using AMPIPROBE technology which incorporates reporter and quencher dyes into the primers. Target detection is based on fluorescence decay when successive rounds of amplification bring fluorophore and quencher-labeled primer pairs in close proximity resulting in FRET. Loss of fluorescence below a defined threshold in a particular channel indicates sample positivity for the corresponding Candida species.
The AMPIPROBE Candida 2 Primer Mix includes primers for a ubiquitously conserved human housekeeping gene (human β-globin). Successful amplification of the internal control serves as an indicator of sample adequacy, extraction efficiency and successful amplification in each individual sample.

Product Specification

Sensitivity:In a validation study approved by the New York State Department of Health, the AMPIPROBE® Candida 2 Assay was determined to have the following percentage of Sensitivity:

Candida parapsilosis - 94.70%
Candida tropicalis - 100.10%
 
Applications:qPCR
 
Application Notes:The AMPIPROBE® Candida 2 Assay is compatible with any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN Rotor-Gene Q
 
Species reactivity:Candida parapsilosis, Candida tropicalis
 
Specificity:In a validation study approved by the New York State Department of Health, the AMPIPROBE® Candida 1 Assay was determined to have the following percentage of Specificity:

Candida parapsilosis - 100.00%
Candida tropicalis - 96.50%
 
Use/Stability:All components are stable at -20°C until the kit's expiration date.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Contents:AMPIGENE® HS Taq DNA Polymerase
AMPIGENE® dNTP Mix
AMPIPROBE® 5X Assay Buffer
AMPIPROBE® Candida 2 Primer Mix
Candida 2 Positive PCR Control
Negative PCR Control
Nuclease-free Water
 
Scientific Background:Vulvovaginal candidiasis (VVC) is second, after bacterial vaginosis, among the many causes of vaginitis, and is diagnosed in up to 40% of women with vaginal complaints in the primary care setting. Approximately 70-75% of women will have at least one symptomatic Candida vulvovaginal infection during their lives. Accurate detection and differentiation of Candida species that cause VVC leads to a significant improvement in clinical management of women with vaginal complaints, especially in women with recurrent VVC (RVVC).
The current assay provides rapid and accurate results for the detection of C. albicans, C. glabrata, and C. krusei DNA in a user-supplied sample of interest.
 

General Literature References

Vulvovaginal candidosis: J.D. Sobel; Lancet 369, 1961 (2007), Abstract;
Evaluation of vaginal complaints: M.R. Anderson, et al.; JAMA 291, 1368 (2004), Abstract;

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