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CYTAG® SuperCGH Labeling Kit

Superior labeling efficiency for low input samples, with greater sensitivity and a simpler workflow
 
ENZ-GEN120-0100 2x100 Reactions Inquire for pricing
 
ENZ-GEN120-0010 2x10 Reactions Inquire for pricing
Do you need bulk/larger quantities?
 
Also available in custom high throughput formats. 
  • Suitable for previously limiting low input samples, including precious biopsy samples
  • Validated for blood, amniotic fluid, postnatal, and oncology samples
  • Provides excellent dynamic analytical range down to 50 ng
  • Shorter workflow has samples ready for hybridization in < 4 hours
  • Superior DLR scores (0.09-0.12) exceeding industry standards
  • Ability to perform total genomic DNA analysis without amplification or complexity reduction
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations.
 
With optimized, proprietary reagents, Enzo's CYTAG® SuperCGH Labeling Kits for oligo arrays produce high quality data using as little as 50 ng of genomic DNA, without a need for pre-amplification for precious low input samples.
 
ENZ-GEN120 HighestSpecificActivity
50 ng DNA samples were labeled with Enzo's CYTAG® SuperCGH Labeling Kit for Oligo Arrays or a leading competitor's kit. Enzo's proprietary labeling technology generates the highest specific activity of labeling for Cy3 and Cy5.
ENZ-GEN120 SuperiorDyeIncorporation
50 ng DNA samples were labeled with Enzo's CYTAG® SuperCGH Labeling Kit for Oligo Arrays or a leading competitor's kits.
ENZ-GEN120 LowestDLRScores
Comparative analysis of 50 ng DNA samples labeled with Enzo's CYTAG® SuperCGH Labeling Kit or that of a leading competitor demonstrates significantly lower DLR scores.
ENZ-GEN120 DynamicAnalyticalRange
CYTAG® SuperCGH labels DNA across a dynamic analytical range
ENZ-GEN120 Prenatal amnios
50 ng DNA input from 4 different amniotic prenatal samples were labeled using CYTAG® SuperCGH Labeling Kit and applied to an 8 x 60K array, showing consistently low DLR scores.
ENZ-GEN120 dup dels
Analysis of patient samples using 50 ng DNA input on oligonucleotide microarray (Agilent 4 x 180K, CGH); A) Patient with Trisomy 9 showing duplication of Chromosome 9 (9p24.2-p11.2): DLRs = 0.149; B) Patient with Jacobsen Syndrome showing deletion of Chromosome 11 (11q24.1-q25): DLRs = 0.123; C) Amniotic sample 3: DLRs = 0.121; D) Amniotic sample 4: DLRs = 0.125
ENZ-GEN120 kit-box
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ENZ-GEN120 HighestSpecificActivity ENZ-GEN120 SuperiorDyeIncorporation ENZ-GEN120 LowestDLRScores ENZ-GEN120 DynamicAnalyticalRange ENZ-GEN120 Prenatal amnios ENZ-GEN120 dup dels ENZ-GEN120 kit-box

Product Specification

Applications:CGH
 
Application Notes:Validated on Agilent SurePrint Microarrays.
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect Super Cyanine 3- and 5-dUTPs from light at all times. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:-0010 size:
ENZ-GEN120-0010: Primers/Reaction Buffer, 1 x 400 µL; Super Cyanine 3-dUTP Nucleotide Mix, 1 x 100 µL; Super Cyanine 5-dUTP Nucleotide Mix, 1 x 100 µL; Klenow DNA Polymerase, 1 x 20 µL; Stop Buffer, 1 x 100 µL; Nuclease-free Water, 1 x 1 mL; PCR & Gel Clean-up columns, 20 tests
-0100 size:
ENZ-GEN120-0100: Primers/Reaction Buffer, 1 x 4 mL; Super Cyanine 3-dUTP Nucleotide Mix, 1 x 1 mL; Super Cyanine 5-dUTP Nucleotide Mix, 1 x 1 mL; Klenow DNA Polymerase, 1 x 0.2 mL; Stop Buffer, 1 x 1 mL; Nuclease-free Water, 1 x 10 mL; PCR & Gel Clean-up columns, 200 tests

PCR & Gel Clean-up Columns can be re-ordered using ENZ-GEN100
 
Scientific Background:
 
Technical Info/Product Notes:Application Note:
Labeling of Small DNA Quantities With CYTAG® SuperCGH Labeling Kit for Prenatal Diagnostics
 

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