The DIGX® HPV type 16/18/31/33/51 probe is a mixture of digoxigenin-labeled HPV type 16, HPV type 18, HPV type 31, HPV type 33 and HPV type 51 specific probes in buffered formamide and hybridization enhancers.
Product Details
Applications:
ISH (in situ hybridization)
Application Notes:
The DIGX® HPV type 16/18/31/33/51 probe employs a mixture of the HPV type 16, HPV type 18, HPV type 31, HPV type 33 and HPV type 51 specific probes that detect HPV types that have been associated with either condyloma or cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIS). The probe mixture is denatured and hybridized to the tissue section fixed and pretreated on the microscope slide. Specific hybridization between the HPV DNA probe and DNA in the specimen is determined by the detection of digoxigenin.
Shipping:
Blue Ice Not Frozen
Long Term Storage:
+4°C
Use/Stability:
Stable for at least one year after receipt when stored as recommended. This product is ready-to-use and additional dilution is not recommended.
Handling:
Allow contents to warm up to room temperature prior to use.
Technical Info/Product Notes:
The HPV probe can be optimized for a polymer detection system using diluent buffer, In situ hybridization buffer for HPV probes (Ready-to-Use) (Prod. No. ENZ-33905).
DIGX® rabbit anti-digoxigenin linker (Prod. No. ENZ-ABS303) or DIGX® mouse anti-digoxigenin linker (Prod. No. ENZ-ABS304) can be used for the detection of the DIGX® HPV probes in conjunction with POLYVIEW® PLUS reagents and kits.
This product or the use of this product is covered by one or more Enzo patents, including, but not limited to the following: U.S. Patent No. 4,994,373; Canadian Patent No. 1,309,672; and patents pending.
Regulatory Status:
RUO - Research Use Only
DIGX® HPV type 16/18/31/33/51 probe was used to assay cervical tissue using a Leica Bond III. Image was acquired with 40x objective.
Workflow of hybridization with DIGX® HPV Probes and detection with DIGX® linkers and POLYVIEW® PLUS reagents
DIGX® HPV type 16/18/31/33/51 probe was used to assay cervical tissue using a Leica Bond III. Image was acquired with 10x objective.
