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Nick translation DNA labeling system 2.0

Nick translation system for preparing FISH probes
 
ENZ-GEN111-0050 50 tests 260.00 USD
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  • Ready-to-use Nick Translation Enzyme Mix minimizes pipetting error
  • Short labeling time - only 1 hour
  • Validated with fluorophore, biotin, and digoxigenin labeled dUTPs
The Nick Translation DNA Labeling System 2.0 provides a simple and efficient method for generating labeled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1kb for fluorescent in situ hybridization (FISH) applications.

The Nick Translation DNA Labeling System 2.0 can accommodate a wide range of fluorophore-labeled, biotin-labeled, and digoxigenin-labeled nucleotides. In addition to choice of label, the kit design allows the user to optimize incorporation and product size by adjusting the ratio of labeled-dUTP to dTTP. The ready-to-use NT Enzyme Mix is user friendly and minimizes error from pipetting.

Probes labeled by nick translation can be used in many different hybridization techniques including: in situ hybridization (ISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and reassociation kinetic studies.
ENZ-GEN111 Hybrid-Red650-Green496
Hybridization of a Red 650 labeled BAC probe to c-Myc and a Green 496 labeled centromere 8 probe to metaphase spread human chromosomes.
ENZ-GEN111 Hybrid-Green496-Orange552
Hybridization of a Green 496 labeled BAC probe to c-Myc and an Orange 552 labeled centromere 8 probe to metaphase spread human chromosomes.
ENZ-GEN111 Biotin-label-HPV-tissue
Hybridization of a biotin-labeled HPV Type 6/11 probe to cervical biopsy tissue. Visualized with streptavidin-AP and HIGHDEF® red IHC chromogen (AP) (ADI-950-140).
ENZ-GEN111 Digx-label-HPV-tissue
Hybridization of a digoxigenin-labeled HPV Type 6/11 probe to cervical biopsy tissue. Visualized with anti-dig-AP and HIGHDEF® red IHC chromogen (AP) (ADI-950-140).
ENZ-GEN111 chromosome
Nick Translation DNA Labeling System 2.0 (ENZ-GEN111) was used to label BAC DNA probe for TP53 with Orange 552 dUTP (ENZ-42842) and BAC DNA probe for Centromere 17 with Green 496 dUTP (ENZ-42831). Labelled probes were hybridized to metaphase spreads. (Institut Universitaire du Cancer Toulouse Oncopole)
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ENZ-GEN111 Hybrid-Red650-Green496 ENZ-GEN111 Hybrid-Green496-Orange552 ENZ-GEN111 Biotin-label-HPV-tissue ENZ-GEN111 Digx-label-HPV-tissue ENZ-GEN111 chromosome

Product Specification

Applications:ISH (in situ hybridization), FISH
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:NT Enzyme Mix 250 µL
Reaction Buffer 250 µL
dNTP Mix (dATP, dGTP, dCTP) 250 µL
dTTP 180 µL
Stop Buffer 250 µL
Nuclease-free Water 5 mL
 
Technical Info/Product Notes:Application Notes:
Assessing the Effects of Humidity on FISH Using the Nick Translation DNA Labeling System 2.0
 
 

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Digoxigenin-dUTP, alkali-stable 

Digoxigenin-labeled nucleotide suitable for non-radioactive labeling of DNA probes
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Superior labeling efficiency and better dye incorporation results in less failed runs
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In situ hybridization buffer (1.25X concentrate) 

Print as PDF
 
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In situ hybridization wash reagent 

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