Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

RsaI

Restriction  enzyme for molecular biology applications
 
ENZ-GEN109-1000 1000 U 61.00 USD
Do you need bulk/larger quantities?
 
  • High quality enzyme with stringent QC testing
  • Includes 10X Cutting Buffer
  • BSA premixed into buffers
  • Suitable for molecular cloning, restriction site mapping, genotyping, southern blotting, SNP
RsaI is a restriction enzyme that recognizes GT^AC sites.

10X Cutting Buffer is included that contains BSA, which enhances enzyme stability and binds to contaminants in DNA preps.

Product Specification

Source:Produced in an E. coli strain that carries the cloned RsaI gene from Rhodopseudomonas sphaeroides.
 
Concentration:RsaI 10,000 units/ml
Includes 10X Cutting Buffer
 
Formulation:Liquid. In 100mM sodium chloride, containing 10mM Tris-HCl, pH 7.4, 1mM DTT, 0.1mM EDTA, 50% glycerol and 200µg/ml BSA.

After reconstitution, 1X Cutting Buffer: 50mM potassium acetate, 20mM Tris-acetate, 10mM magnesium acetate, 100µg/ml BSA, pH 7.9, at 25°C.
 
Quality Control:Exonuclease Activity (Radioactivity Release):
A 50 µl reaction in 1X Cutting Buffer containing 1 µg of a mixture of single and double stranded [3H] E. coli DNA and a minimum of 50 units of RsaI incubated for 4 hours at 37°C releases <0.1% of the total radioactivity.

Ligation and Recutting (Terminal Integrity):
After a 10-fold over digestion of λ DNA with RsaI, ~95% of the DNA fragments can be ligated with T4 DNA ligase in 16 hours at 16°C. Of these ligated fragments, >95% can be recut with RsaI.

Non-Specific DNase Activity (16 Hour):
A 50 µl reaction in 1X Cutting Buffer containing 1 µg of λ DNA and a minimum of 50 Units of RsaI incubated for 16 hours at 37°C results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis.
 
Activity:One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
 
Applications:Southern Blot, Cloning, Genotyping, CGH
 
Application Notes:For molecular cloning, restriction site mapping, genotyping, Southern blotting, SNP.
 
Reconstitution:Reconstitute 10X Cutting Buffer with nuclease-free water
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Protocol:Reaction conditions:
1X Cutting Buffer 37°C
 
ENZ-GEN109 kitbox
Please mouse over
ENZ-GEN109 kitbox

Related Products

CYTAG® CGH Labeling kit 

Superior labeling efficiency and better dye incorporation results in less failed runs
CGH | Print as PDF
 
ENZ-42671-K010 2x10 Reactions Inquire for pricing
 
ENZ-42671-K100 2x100 Reactions Inquire for pricing
Do you need bulk/larger quantities?
 

CGH labeling kit for BAC arrays 

CGH | Print as PDF
 
ENZ-42670 20 Reactions Inquire for pricing
Do you need bulk/larger quantities?
 

AluI 

Restriction  enzyme for molecular biology applications
Produced in an E. coli strain that carries the cloned AluI gene from Arthrobacter luteus., Southern Blot, Cloning, Genotyping, CGH | Print as PDF
 
ENZ-GEN108-1000 1000 U 82.00 USD
Do you need bulk/larger quantities?
 

CYTAG® TotalCGH Labeling kit 

Complete kit to digest and label DNA for CGH+SNP arrays
CGH | Print as PDF
 
ENZ-42674-0010 2x10 Reactions Inquire for pricing
 
ENZ-42674-0100 2x100 Reactions Inquire for pricing
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
RsaI
By biological activity:
RsaI Enzyme
RsaI Recombinant protein
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,