| ENZ-GEN109-1000 | 1000 U | 76.00 USD |  |
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| Source: | Produced in an E. coli strain that carries the cloned RsaI gene from Rhodopseudomonas sphaeroides. |
| Concentration: | RsaI 10,000 units/ml Includes 10X Cutting Buffer |
| Formulation: | Liquid. In 100mM sodium chloride, containing 10mM Tris-HCl, pH 7.4, 1mM DTT, 0.1mM EDTA, 50% glycerol and 200µg/ml BSA. After reconstitution, 1X Cutting Buffer: 50mM potassium acetate, 20mM Tris-acetate, 10mM magnesium acetate, 100µg/ml BSA, pH 7.9, at 25°C. |
| Quality Control: | Exonuclease Activity (Radioactivity Release): A 50 µl reaction in 1X Cutting Buffer containing 1 µg of a mixture of single and double stranded [3H] E. coli DNA and a minimum of 50 units of RsaI incubated for 4 hours at 37°C releases <0.1% of the total radioactivity. Ligation and Recutting (Terminal Integrity): After a 10-fold over digestion of λ DNA with RsaI, ~95% of the DNA fragments can be ligated with T4 DNA ligase in 16 hours at 16°C. Of these ligated fragments, >95% can be recut with RsaI. Non-Specific DNase Activity (16 Hour): A 50 µl reaction in 1X Cutting Buffer containing 1 µg of λ DNA and a minimum of 50 Units of RsaI incubated for 16 hours at 37°C results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. |
| Activity: | One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. |
| Applications: | Southern Blot, Cloning, Genotyping, CGH |
| Application Notes: | For molecular cloning, restriction site mapping, genotyping, Southern blotting, SNP. |
| Reconstitution: | Reconstitute 10X Cutting Buffer with nuclease-free water |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Protocol: | Reaction conditions: 1X Cutting Buffer 37°C |
| Regulatory Status: | RUO - Research Use Only |
CYTAG® CGH Labeling kit |
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| Superior labeling efficiency and better dye incorporation results in less failed runs | ||||||
| CGH | Print as PDF | ||||||
| ENZ-42671-K010 | 2x10 Reactions | 1,012.00 USD | |
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| ENZ-42671-K100 | 2x100 Reactions | 7,423.00 USD | |
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CGH labeling kit for BAC arrays |
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| CGH | Print as PDF | ||||||
| ENZ-42670 | 20 Reactions | 997.00 USD | |
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AluI |
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| Restriction enzyme for molecular biology applications | ||||||
| Produced in an E. coli strain that carries the cloned AluI gene from Arthrobacter luteus., Southern Blot, Cloning, Genotyping, CGH | Print as PDF | ||||||
| ENZ-GEN108-1000 | 1000 U | 109.00 USD | |
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CYTAG® TotalCGH Labeling kit |
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| Complete kit to digest and label DNA for CGH+SNP arrays | ||||||
| CGH | Print as PDF | ||||||
| ENZ-42674-0010 | 2x10 Reactions | 1,166.00 USD | |
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| ENZ-42674-0100 | 2x100 Reactions | 7,736.00 USD | |
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