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CYGREEN® Nucleic Acid Dye

Nucleic acid dye for gel staining and higher sensitivity qPCR
 
ENZ-GEN105-0100 100 µl 110.00 USD
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  • Higher sensitivity allows detection of low target concentration
  • Greater PCR efficiency for accurate and reproducible results
  • Soluble in water for higher stability
  • Compatible with various enzyme systems
  • Suitable for commonly used rotor-type and plate-type qPCR platforms
  • Validated for qPCR applications and gel staining
  • Lower cost per assay compared to commonly used dyes
CYGREEN® Nucleic Acid Dye is a DNA intercalating agent that is used to stain DNA. The DNA-dye complex emits a fluorescence spectra that makes it suitable for qPCR and gel staining applications.

CYGREEN® Nucleic Acid Dye has excitation/emission spectra similar to commonly used qPCR dyes and can be substituted in to increase sensitivity and reduce cost.

Product Specification

Concentration:10,000x
 
Formulation:Solution in DMSO.
 
Excitation maximum:489nm
 
Emission maximum:515nm
 
Purity:≥93% (HPLC)
 
Appearance:Reddish orange liquid.
 
Applications:qPCR, Gel staining, Melting curve
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-20°C
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
CYGREEN® Nucleic Acid Dye Amplification
CYGREEN® Nucleic acid dye and 4 other competitor dyes were used in qPCR reactions with varying target concentrations. CYGREEN® Nucleic acid dye has higher sensitivity than competitor dyes and was able to detect low target concentrations.
CYGREEN® Nucleic Acid Dye CostComp
qPCR cost comparison between CYGREEN® Nucleic acid dye and four leading competitors. Costs are based on the working concentrations using 25µl reaction size for 100 reactions. For a true comparison, each individual dye was titrated to determine the optimal working concentration. CYGREEN® Nucleic acid dye is the most economical dye for qPCR applications.
CYGREEN® Nucleic Acid Dye CompPlots
CYGREEN® Nucleic acid dye and 4 other competitor dyes were used in qPCR reactions with varying target concentrations. CYGREEN® Nucleic acid dye has higher sensitivity than competitor dyes.
CYGREEN® Nucleic Acid Dye Kit box image
CYGREEN® Nucleic Acid Dye AppendixD
DNA Staining before gel electrophoresis. AMPIGENE® DNA Ladder 100-10,000 bp (ENZ-GEN104) was stained with a 1/10 dilution of a 100x solution of CYGREEN® Nucleic Acid Dye (for a 10x final concentration of CYGREEN® Dye) at room temp for 10 min. 15μL of the stained DNA ladder was loaded into each of 3 wells of a 1% agarose gel. Gel was run for 1h at 100V. CYGREEN® Nucleic acid dye prestained DNA can be visualized in a gel.
CYGREEN® Nucleic Acid Dye AppendixC
10μL of CYGREEN® Nucleic Acid Dye was added to 100mL of a 1% Agarose solution. Gel was poured. 15μL of AMPIGENE® DNA Ladder 100-10,000 bp (ENZ-GEN104) was loaded into three lanes of the gel and was run in TAE buffer for 1h at 100v. CYGREEN® Nucleic Acid precast gel showsbright staining.
CYGREEN® Nucleic Acid Dye Gel
5, 10 and 15µL of AMPIGENE® DNA Ladder 100-10,000 bp (Prod. No. ENZ-GEN104) were loaded onto a 1% Agarose gel in TAE buffer and run 1h at 100v. Gel was sectioned and stained for 1h with CYGREEN® Dye or with 3 other competitors. CYGREEN® Dye shows bright gel staining equivalent or better than competitors.
CYGREEN® Nucleic Acid Dye Excitation and emission
Excitation and Emission Spectra of CYGREEN® Nucleic acid dye bound to DNA. CYGREEN® nucleic acid dye shares spectral properties with other commonly used qPCR dyes, allowing detection in the FAM (or similar) channel.
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CYGREEN® Nucleic Acid Dye Amplification CYGREEN® Nucleic Acid Dye CostComp CYGREEN® Nucleic Acid Dye CompPlots CYGREEN® Nucleic Acid Dye Kit box image CYGREEN® Nucleic Acid Dye AppendixD CYGREEN® Nucleic Acid Dye AppendixC CYGREEN® Nucleic Acid Dye Gel CYGREEN® Nucleic Acid Dye Excitation and emission

Product Literature References

A drug-repositioning screen for primary pancreatic ductal adenocarcinoma cells identifies 6-thioguanine as an effective therapeutic agent for TPMT-low cancer cells: I. Kim, et al.; Mol. Oncol. 12, 1526 (2018), Abstract; Full Text

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