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EQ0 NHS ester

Amine-reactive quencher dye
 
ENZ-CHM208-0001 1 mg 97.00 USD
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  • Commonly used in molecular biology FRET applications to quench fluorophores
  • NHS ester quencher dye can be conjugated to amine-labeled probes
  • Enable multiplexing of probes with the use of different reporter-quencher dye pairs
  • Compatible with most common commercially available fluorophores
EQ0 NHS ester is an amine-reactive quencher dye. It has a broad visible absorption but no detectable fluorescence emission, making it useful as an acceptor in fluorescence  resonance energy transfer (FRET) applications.

It can be used to quench reporter dyes: AMCA, AMCA-X, DEAC, DEAC-X, MCA, MCC, CF™ 350, CF™405S, CF™405M, Pacific Blue™, Cascade Blue®, Marina Blue®, Alexa Fluor® 350, Alexa Fluor® 405, DyLight® 350, DyLight® 405, ATTO 390, ATTO 425, ATTO 465, iFluor™350, iFLuor™405, BD Horizon V450, BUV395, eFluor®450.

Product Specification

MW:439.5
 
Absorbance maximum:445 nm
 
Emission maximum:none
 
Extinction Coefficient:23,600 M-1cm-1
 
Correction Factor (260 nm):0.48
 
Correction Factor (280 nm):0.42
 
Purity:≥80% (HPLC)
 
Appearance:Red brown solid.
 
Solubility:Soluble in DMSO or DMF.
 
Applications:Fluorescent detection
FRET
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.
 
Handling:Protect from moisture. Protect from light.
 
Scientific Background:The EQ dyes are a family of non-fluorescent dyes that are designed for fluorescence quenching in FRET applications.
 
ENZ-CHM208 absorbance
ENZ-CHM208 EQDye Absorbance
Normalized absorbance of EQ dyes showing their range of quenching through FRET.
ENZ-CHM208 Kit Box
ENZ-CHM208 quencher-dye-pair
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ENZ-CHM208 absorbance ENZ-CHM208 EQDye Absorbance ENZ-CHM208 Kit Box ENZ-CHM208 quencher-dye-pair

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EQ1 NHS ester 

Amine-reactive quencher dye
≥80% (HPLC), Fluorescent detection, FRET | Print as PDF
 
ENZ-CHM165-0001 1 mg 97.00 USD
Do you need bulk/larger quantities?
 

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