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EQ2 NHS ester

Amine-reactive quencher dye
ENZ-CHM166-0001 1 mg 102.00 USD
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  • Commonly used in molecular biology FRET applications to quench fluorophores
  • NHS ester quencher dye can be conjugated to amine-labeled probes
  • Enable multiplexing of probes with the use of different reporter-quencher dye pairs
  • Compatible with most common commercially available fluorophores
EQ2 NHS ester is an amine-reactive quencher dye. It has a broad visible absorption but no detectable fluorescence emission, making it useful as an acceptor in fluorescence resonance  energy transfer (FRET) applications.

It can be used to quench reporter dyes: TET, JOE, VIC, HEX, Cy3, NED, TAMRA, Cy3.5, ROX, Texas Red, Cy5, Quasar® 570, Pulsar® 650, CAL Fluor® Gold 540, CAL Fluor® Orange 560, CAL Fluor® Red 590, CAL Fluor® Red 610, CAL Fluor® Red 635, Alexa Fluor® 532, Alexa Fluor® 546, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 594, Alexa Fluor® 610, Alexa Fluor® 633, Alexa Fluor® 647, DyLight® 550, DyLight® 594, DyLight® 633, DyLight® 650, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 550, ATTO 565, ATTO Rho11, ATTO 590, ATTO 594, ATTO 633, ATTO Rho14, and ATTO 647.

Product Specification

Excitation maximum:583 nm
Emission maximum:none
Extinction Coefficient:37,000 M-1cm-1
Correction Factor (260 nm):0.49
Correction Factor (280 nm):0.40
Purity:≥80% (HPLC)
Appearance:Dark purple solid.
Solubility:Soluble in DMSO or DMF.
Applications:Fluorescent detection
Shipping:Shipped on Dry Ice
Long Term Storage:-20°C
Use/Stability:Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.
Handling:Protect from moisture. Protect from light.
Scientific Background:The EQ dyes are a family of non-fluorescent dyes that are designed for fluorescence quenching in FRET applications.
ENZ-CHM166 adsorbance
Absorbance spectra
ENZ-CHM166 EQDye Absorbance
Normalized absorbance of EQ dyes showing their range of quenching through FRET.
ENZ-CHM166 Kit Box
ENZ-CHM166 quencher-dye-pair
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ENZ-CHM166 adsorbance ENZ-CHM166 EQDye Absorbance ENZ-CHM166 Kit Box ENZ-CHM166 quencher-dye-pair

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