Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

EQ1 NHS ester

Amine-reactive quencher dye
 
ENZ-CHM165-0001 1 mg 97.00 USD
Do you need bulk/larger quantities?
 
  • Commonly used in molecular biology FRET applications to quench fluorophores
  • NHS ester quencher dye can be conjugated to amine-labeled probes
  • Enable multiplexing of probes with the use of different reporter-quencher dye pairs
  • Compatible with most common commercially available fluorophores
EQ1 NHS ester is an amine-reactive quencher dye. It has a broad visible absorption but no detectable fluorescence emission, making it useful as an acceptor in fluorescence  resonance energy transfer (FRET) applications.

It can be used to quench reporter dyes: FAM, Cy2, TET, JOE, VIC, HEX, Cy3, NED, TAMRA, Cy3.5, ROX, Texas Red, Quasar® 570, CAL Fluor® Gold 540, CAL Fluor® Orange 560, CAL Fluor® Red 590, CAL Fluor® Red 610, Alexa Fluor® 430, Alexa Fluor® 488, Alexa Fluor® 532, Alexa Fluor® 546, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 594, DyLight® 488, DyLight® 550, DyLight® 594, ATTO 488, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 550, ATTO 565, ATTO Rho11, ATTO 590, ATTO 594.

Product Specification

MW:535.5
 
Absorbance maximum:535 nm
 
Emission maximum:none
 
Extinction Coefficient:39,200 M-1cm-1
 
Correction Factor (260 nm):0.34
 
Correction Factor (280 nm):0.27
 
Purity:≥80% (HPLC)
 
Appearance:Dark red solid.
 
Solubility:Soluble in DMSO or DMF.
 
Applications:Fluorescent detection
FRET
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.
 
Handling:Protect from moisture. Protect from light.
 
Scientific Background:The EQ dyes are a family of non-fluorescent dyes that are designed for fluorescence quenching in FRET applications.
 
ENZ-CHM165 adsorbance
ENZ-CHM165 EQDye Absorbance
Normalized absorbance of EQ dyes showing their range of quenching through FRET.
ENZ-CHM165 Kit Box
ENZ-CHM165 quencher-dye-pair
Please mouse over
ENZ-CHM165 adsorbance ENZ-CHM165 EQDye Absorbance ENZ-CHM165 Kit Box ENZ-CHM165 quencher-dye-pair

Related Products

EQ2 NHS ester 

Amine-reactive quencher dye
≥80% (HPLC), Fluorescent detection, FRET | Print as PDF
 
ENZ-CHM166-0001 1 mg 97.00 USD
Do you need bulk/larger quantities?
 

EQ0 NHS ester 

Amine-reactive quencher dye
≥80% (HPLC), Fluorescent detection, FRET | Print as PDF
 
ENZ-CHM208-0001 1 mg 97.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
DNA
RNA
Protein
By biological activity:
DNA Dye
RNA Dye
Protein Dye
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

Product Flyers
EQ Quencher Dyes for FRET Applications
EQ Quencher Dyes for FRET Applications
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,