- Commonly used in molecular biology FRET applications to quench fluorophores
- NHS ester quencher dye can be conjugated to amine-labeled probes
- Enable multiplexing of probes with the use of different reporter-quencher dye pairs
- Compatible with most common commercially available fluorophores
EQ1 NHS ester is an amine-reactive quencher dye. It has a broad visible absorption but no detectable fluorescence emission, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.
It can be used to quench reporter dyes: FAM, Cy2, TET, JOE, VIC, HEX, Cy3, NED, TAMRA, Cy3.5, ROX, Texas Red, Quasar® 570, CAL Fluor® Gold 540, CAL Fluor® Orange 560, CAL Fluor® Red 590, CAL Fluor® Red 610, Alexa Fluor® 430, Alexa Fluor® 488, Alexa Fluor® 532, Alexa Fluor® 546, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 594, DyLight® 488, DyLight® 550, DyLight® 594, ATTO 488, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 550, ATTO 565, ATTO Rho11, ATTO 590, ATTO 594.
Product Details
| MW: | 535.5 |
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| Excitation maximum: | 535 nm |
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| Emission maximum: | none |
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| Extinction Coefficient: | 39,200 M-1cm-1 |
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| Correction Factor (260 nm): | 0.34 |
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| Correction Factor (280 nm): | 0.27 |
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| Purity: | ≥80% (HPLC) |
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| Appearance: | Dark red solid. |
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| Solubility: | Soluble in DMSO or DMF. |
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| Applications: | Fluorescent detection
FRET
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. |
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| Handling: | Protect from moisture. Protect from light. |
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| Scientific Background: | The EQ dyes are a family of non-fluorescent dyes that are designed for fluorescence quenching in FRET applications. |
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| Regulatory Status: | RUO - Research Use Only |
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Normalized absorbance of EQ dyes showing their range of quenching through FRET.
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