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NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)

Cell permeable DNA stain that can be used for a wide range of applications
 
ENZ-CHM103-0200 200 µl 201.00 USD
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  • This blue fluorescent specific DNA dye is stable and has high purity
  • Live, permeable and fixed cells can be analyzed
  • No photobleaching effect
  • No RNase treatment is required
  • Multiplex with GFP and red dyes
  • Validated for a wide range of cell densities
  • Quick and easy to use!
The NUCLEAR-ID® Blue DNA stain is a cell permeable dye, designed for use in a range of fluorescence detection technologies, in the discrimination of nucleated cells. It is resistant to photobleaching and is suitable for live-cell staining of nuclei. Also this dye provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry. Potential applications of this reagent for live-cell studies are in the determination of cellular DNA content and cell cycle distribution, for the detection of variations in growth patterns, for monitoring apoptosis, and for evaluating tumor cell behavior and suppressor gene mechanisms.

Product Specification

Quantity:200µl
 
Absorbance maximum:350nm
 
Emission maximum:461nm
 
Purity:≥95% (HPLC)
 
Appearance:Frozen liquid.
 
Applications:Flow Cytometry, Fluorescent detection
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:The NUCLEAR-ID® Blue DNA stain is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
Protocol:Live Cell Staining with NUCLEAR-ID® Blue DNA Dye for Nuclear Visualization by Fluorescence/Confocal Microscopy

Reagent Preparation:
Mix 1μl of NUCLEAR-ID® Blue DNA Dye in 1 ml of buffer of choice. This volume is sufficient for 10 assays and may be scaled according to need.

Staining Adherent cells:
  1. Grow cells directly onto glass slides or polystyrene tissue culture plates until ~80% confluent via standard tissue culture practices.
  2. Remove growth media.
  3. Dispense the freshly diluted staining solution in a volume sufficient for covering the cell monolayer.
  4. Protect samples from light and incubate for 30 minutes at 37°C.
  5. Remove the excess staining solution and, if necessary, add a few drops of buffer to prevent the cells from drying out.
  6. Cover cells with a glass cover slip and observe under a fluorescence/confocal microscope with a filter for DAPI (Ex/Em: 350/470nm).
Staining Non-Adherent Cells:
  1. Grow cells via standard tissue culture practices.
  2. Collect about 1 x 105cells. Centrifuge at 500 x g for 5 minutes. Remove supernatant.
  3. Re-suspend cells in a volume of the freshly diluted staining solution sufficient for covering the cell pellet.
  4. Protect samples from light and incubate for 30 minutes at 37°C.
  5. Centrifuge at 500 x g for 5 minutes. Remove supernatant.
  6. Re-suspend cells in 100µl buffer.
  7. Plate 10-15µl of cells on a glass slide.
  8. Cover cells with a glass cover slip and observe under a fluorescence/confocal microscope with a filter for DAPI (Ex/Em: 350/470nm).
 

General Literature References

DNA measurement and cell cycle analysis by flow cytometry: R. Nunez; Curr. Issues Mol. Biol. 3, 67 (2001), Abstract;
Flow Cytometry. Methods in Cell Biology: Darzynkiewicz, Robinson and Crissman (eds.); 41 & 42, (1994), Book,

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