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Chromogranin A (human) monoclonal antibody (PHE5)

 
ENZ-C34932 0.5 ml ~10 µg 226.00 USD
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Alternative size available: ENZ-30932 (6ml)

Product Specification

Alternative Name:Neuroendocrine, CgA
 
Clone:PHE5
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Human pheochromocytoma.
 
UniProt ID:P10645
 
Species reactivity:Human
 
Specificity:PHE5 is specific to chromogranin A, a highly acidic protein associated with the “dense core” granules of many cells of the neural and neuroendocrine system. It reacts with neuroendocrine cells of the gut, pancreatic islet and adrenal medulla, but does not react with any cells in the brain or with melanoma cells. The antibody stains carcinoid tumors, pheochromocytomas and paragangliomas. It is nonreactive with carcinomas, lymphomas and sarcomas.
 
Applications:IHC (PS)
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections; this antibody can be used for staining of paraffin-embedded tissue sections fixed in neutral buffered formalin and is compatible with most commonly used histological fixatives. Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection). A 20- to 50-fold dilution of the antibody is suggested. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually.
 
Purity Detail:Affinity purified.
 
Formulation:Liquid. In PBS containing 0.1% sodium azide and 1% BSA.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
ENZ-C34932 5x
Immunohistochemistry analysis of human endocrine pancreas tissue stained with chromogranin A, mAb (PHE5). Original magnifications: 5x and 63x, respectively.
ENZ-C34932 63x
Immunohistochemistry analysis of human endocrine pancreas tissue stained with chromogranin A, mAb (PHE5). Original magnifications: 5x and 63x, respectively.
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ENZ-C34932 5x ENZ-C34932 63x

Product Literature References

Comparative study of seven neuroendocrine markers in pancreatic endocrine tumours: C. Bordi, et al.; Virchows Arch. A Pathol. Anat. Histopathol. 413, 387 (1988), Abstract;
Distribution of chromogranin A and secretogranin I (chromogranin B) in neuroendocrine cells and tumors: R.V. Lloyd, et al.; Am. J. Pathol. 130, 296 (1988), Abstract; Full Text
PHE5, a new monoclonal antibody to a unique neuroendocrine granule protein: K. Riddell, et al.; Lab. Invest. 56, 64A (1987),

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Chromogranin A (human) monoclonal antibody (PHE5) (Ready-to-Use) 

IHC (PS) | Print as PDF
 
ENZ-30932 6 ml ~6 µg 194.00 USD
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