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Prestained Protein Ladder (3.5 - 245 kDa)

Protein ladder for use with protein gel electrophoresis
 
ENZ-ACC131-0500 500 µl 119.00 USD
Do you need bulk/larger quantities?
 
  • 3 µL or 5 µL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
  • Three color protein standard with 13 prestained proteins.
  • Apply more for thicker (>1.5 mm) or larger gel.
Enzo's Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

Our Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute or add reducing agent before loading.

Product Specification

Formulation:Approximately 0.1~0.4 mg/ml of each protein in the buffer (20mM Tris-phosphate, pH 7.5 at 25°C), 2% SDS, 10mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol).
 
Applications:SDS-PAGE
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Use/Stability:Store at 4°C for 3 months. Store at -20°C for 24 months.
 
Prestained Protein Ladder (3.5 - 245 kDa) Migration patterns
Guide for Molecular Weight Estimation (kDa): Migration patterns of Prestained Protein Ladder in different electrophoresis conditions.
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Prestained Protein Ladder (3.5 - 245 kDa) Migration patterns

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