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HIGHDEF® Green AP Chromogen/Substrate

High definition IHC chromogen producing brilliant green color for AP-activated development.
 
ENZ-ACC130-0030 30 ml Inquire for pricing
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HIGHDEF® Green AP Chromogen/Substrate is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridization systems.
HIGHDEF Green AP Chromogen/Substrate IHC cervical tissue Ki-67
Cervical tissue incubated with a primary antibody to Ki-67, stained with POLYVIEW® PLUS (anti-Mouse) Reagent and HIGHDEF® Green AP Chromogen/Substrate
HIGHDEF Green AP Chromogen/Substrate IHC CD44
CD44 stained with SAVIEW® PLUS AP Reagent (ENZ-ACC111) and HIGHDEF® Green AP Chromogen/Substrate.
HIGHDEF Green AP Chromogen/Substrate IHC human tonsil multiplex
HIGHDEF Green AP Chromogen/Substrate IHC human tonsil multiplex 2
Multiplex IHC staining performed on formalin-fixed paraffin-embedded human tonsil stained with CD8 antibody labeled with HIGHDEF® black IHC chromogen (HRP) (Prod. Ref. ADI-950-171), CD68 antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. Ref. ADI-950-170), CD163 antibody labeled with HIGHDEF® green AP chromogen/substrate (Prod. Ref. ENZ-ACC130), and hematoxylin counterstain. Image used with permission from Plateforme de Pathologie Expérimentale et Translationnelle - PETRA, Unité AMMICa, Gustave Roussy Cancer Campus, Villejuif, France.
HIGHDEF Green AP Chromogen/Substrate IHC Mousebrain b-amyloid-p-tau
Dual IHC staining performed on formalin-fixed paraffin-embedded tissue section from human brain with Alzheimer’s disease. The tissue sections were stained for alpha-amyloid with POLYVIEW® PLUS AP (anti-rabbit) reagent (Prod. No. ENZ-ACC110) and HIGHDEF® Green AP Chromogen/Substrate (Prod. No. ENZ-ACC130), and phospho-tau with POLYVIEW® PLUS HRP (anti-mouse) reagent (Prod. No. ENZ-ACC104) and HIGHDEF DAB Chromogen/Substrate Set (Prod. No. ENZ-ACC105). Picture courtesy of Dr. Karelle Leroy Laboratoire d’histologie, de neuroanatomie et de neuropathologie, Université Libre de Bruxelles, Bruxelles, Belgium.
HIGHDEF Green AP Chromogen/Substrate IHC ADbrain b-amyloid-p-tau
Dual IHC staining performed on formalin-fixed paraffin-embedded tissue section from transgenic mouse brain expressing human mutated APP and PS1. The tissue sections were stained for alpha-amyloid with POLYVIEW® PLUS AP (anti-rabbit) reagent (Prod. No. ENZ-ACC110) and HIGHDEF® Green AP Chromogen/Substrate (Prod. No. ENZ-ACC130), and phospho-tau with POLYVIEW® PLUS HRP (anti-mouse) reagent (Prod. No. ENZ-ACC104) and HIGHDEF DAB Chromogen/Substrate Set (Prod. No. ENZ-ACC105). Picture courtesy of Dr. Karelle Leroy Laboratoire d’histologie, de neuroanatomie et de neuropathologie, Université Libre de Bruxelles, Bruxelles, Belgium.
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HIGHDEF Green AP Chromogen/Substrate IHC cervical tissue Ki-67 HIGHDEF Green AP Chromogen/Substrate IHC CD44 HIGHDEF Green AP Chromogen/Substrate IHC human tonsil multiplex HIGHDEF Green AP Chromogen/Substrate IHC human tonsil multiplex 2 HIGHDEF Green AP Chromogen/Substrate IHC Mousebrain b-amyloid-p-tau HIGHDEF Green AP Chromogen/Substrate IHC ADbrain b-amyloid-p-tau

Product Specification

Applications:IHC
ISH (in situ hybridization)
 
Contents:HIGHDEF® Green AP Chromogen, HIGHDEF® Green AP Substrate
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:The HIGHDEF® Green AP Chromogen/Substrate is a substrate-chromogen system designed to be used for either IHC or ISH when utilizing alkaline phosphatase. It has been modified to increase stability and staining intensity, producing a strong green color that is insoluble in alcohol and xylene substitutes (both aliphatic hydrocarbon and citrus based); therefore sections can be dehydrated in alcohol, cleared in xylene substitute, and permanently mounted. However, we recommend air drying slides and then permanently mounting. This chromogen/substrate system may be used for both automation and manual use.
 

Product Literature References

NAMPT signaling is critical for the proangiogenic activity of tumor‐associated neutrophils: E. Pylaeva, et al.; Int. J. Cancer 144, 136 (2019), Abstract;

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Ready-to-use histologic stain with enhancers
IHC, ISH (in situ hybridization) | Print as PDF
 
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POLYVIEW® PLUS AP (anti-rabbit) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
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