Streptavidin-based detection with reduced carbohydrate and biotin background
Suitable for both manual and automated platforms
Streptavidin Blocker/Diluent further reduces background (sold separately)
5-10X more sensitive than avidin based detection
Customize with HIGHDEF® chromogens of your choice (sold separately)
SAVIEW® PLUS AP Reagent is a ready-to-use streptavidin-based nanopolymer detection reagent suitable for immunohistochemical detection of antigens in formalin-fixed paraffin-embedded (FFPE) tissues and frozen sections. The detection reagent may also be used with blood smears, cytosmears, and cell preparations. In addition, the SAVIEW® PLUS AP Reagent can be used for in situ hybridization detection of biotinylated DNA/RNA probes.
SAVIEW® PLUS AP (Enzo Life Sciences) was used to perform ISH: Manual method. Sections were stained with HIGHDEF® Green AP Chromogen/Substrate and counterstained with HIGHDEF® Hematoxylin. Images acquired under 10X objective.
Mouse anti-CD44 and SAVIEW® PLUS AP (Enzo Life Sciences) were used to perform IHC: Manual method. Sections were stained with HIGHDEF® Green AP Chromogen/Substrate and counterstained with HIGHDEF® Hematoxylin. Images acquired under 10X objective.
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Product Details
Assay Time:
< 2 hours
Applications:
IHC ISH (in situ hybridization)
Application Notes:
IHC
ISH (in situ hybridization)
Quantity:
150 tests × 150 µL (22.5 mL total volume)
Handling:
Do not freeze.
Shipping:
Blue Ice
Long Term Storage:
+4°C
Technical Info/Product Notes:
The SAVIEW® PLUS AP Reagent has been developed by labeling streptavidin molecules with a nanopolymer of alkaline phosphatase (AP) using proprietary technology. This ensures consistent and reproducible detection of biotinylated primary/secondary antibodies or biotinylated nucleic acid probes on tissue sections.
The Streptavidin Blocker/Diluent (ENZ-ACC109) further reduces background and is suitable for diluting the SAVIEW® PLUS AP Reagent.
