Replaces Prod. #: ADI-950-245
The Antibody Blocker/Diluent can be used to block nonspecific staining due to antibodies during immunostaining. It can also be used as a diluent for the POLYVIEW® PLUS detection reagents or as a primary antibody diluent.
This product is intended for immunohistochemistry and in situ hybridization with formalin-fixed parafiin-embedded tissue sections and frozen sections. The blocker/diluent may also be used with blood smears, cytosmears, and cell preparations.
Product Details
| Applications: | IHC
ISH (in situ hybridization)
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Technical Info/Product Notes: | The Antibody Blocker/Diluent is a unique blocking solution used to reduce nonspecific background staining. It is a universal blocking agent which eliminates the need to match blocker to antibody species. In addition to immunohistochemistry, the blocker/diluent can also be used for in situ hybridization.
The Antibody Blocker/Diluent can also be used as a diluent for POLYVIEW® PLUS HRP (anti-rabbit) reagent (ENZ-ACC103), POLYVIEW® PLUS HRP (anti-mouse) reagent (ENZ-ACC104), and POLYVIEW® PLUS AP (anti-rabbit) reagent (ENZ-ACC110). It can also serve as a diluent for the primary antibody. |
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| Protocol: |
Recommended protocol for IHC (immunohistochemistry)
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Arrange slides of formalin fixed paraffin-embedded (FFPE) tissue sections in a slide holder.
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Heat in drying oven at 55-60°C for 20 minutes.
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Deparaffinize with xylene or xylene substitute for 10 minutes.
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Follow with 100% ethanol for 6 minutes.
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Rehydrate slides for one minute in each step - 90%, 70%, 50% ethanol and in distilled or deionized water.
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Incubate the tissue with peroxidase block for 5 minutes.
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Wash with PBST for 2 minutes.
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Retrieve antigen with Antigen Retrieval Reagent (Citrate, pH 6) or Antigen Retrieval Reagent (Tris-EDTA, pH 9) for 20 minutes at 99°C.
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Wash for 5 minutes with PBST.
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Wipe the excess liquid around the section on the glass slide and encircle the tissue section with a PAP pen.
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Dry slide for 10 seconds.
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Incubate sections with Antibody Buffer/Diluent for 10 minutes to block non-specific antibody binding.
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Researcher needs to optimize the concentrations and incubation times for primary antibodies (recommended 20 - 30 minutes)
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Wash for 5 min with PBST.
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After adding primary antibody, use POLYVIEW® PLUS Detection reagents and HIGHDEF® Chromogens to visualize antigens.
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
H-Ferritin Produced by Myeloid Cells Is Released to the Circulation and Plays a Major Role in Liver Iron Distribution during Infection: A.C. Moreira, et al.; Int. J. Mol. Sci.
2, 269 (2022),
Abstract;
Comprehensive Characterization of Immunological Profiles and Clinical Significance in Hepatocellular Carcinoma: P. Tao, et al.; Front. Oncol.
10, 574778 (2021),
Abstract;
Full Text
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