Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

HIGHDEF® DAB Chromogen/Substrate Set

Brown chromogen for use with HRP immunohistochemistry staining systems
 
ENZ-ACC105-0200 200 ml Inquire for pricing
Do you need bulk/larger quantities?
 
Replaces Prod. #: ADI-950-211

Available from stock, Ready for shipment.
  • Strong, clear color development
  • Compatible with high-throughput systems
The HIGHDEF® DAB Chromogen/Substrate Set can be used in conjunction with peroxidase-based immunostaining systems. Peroxidase from the antibody detection system reacts with hydrogen peroxide substrate to degrade it, which then reacts with DAB, precipitating it at positive sites and yielding a dark brown color.
ENZ-ACC105 IHC-1
Cancerous tonsil tissue was incubated with an anti-Ki-67 primary antibody and then stained with POLYVIEW® PLUS HRP (anti-Rabbit) Reagent (Cat. No. ENZ-ACC104) and then color was developed using HIGHDEF® DAB Chromogen/Substrate Set.
ENZ-ACC105 IHC-2
Cancerous tonsil tissue was incubated with an anti-Ki-67 primary antibody and then stained with POLYVIEW® PLUS HRP (anti-Rabbit) Reagent (Cat. No. ENZ-ACC104) and then color was developed using HIGHDEF® DAB Chromogen/Substrate Set.
Please mouse over
ENZ-ACC105 IHC-1 ENZ-ACC105 IHC-2

Product Specification

Applications:IHC
ISH (in situ hybridization)
 
Kit/Set Contains:HIGHDEF® DAB Chromogen, HIGHDEF® DAB Substrate Buffer
 
Quantity:Materials provided for 1,000 tests
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:3,3'-Diaminobenzidine (DAB), a widely used chromogen for immunoperoxidase staining, is well accepted among pathologists because of its increased sensitivity and decreased background when compared to amino ethyl carbazole (AEC). Specimens stained with DAB can be dehydrated, cleared, and mounted for permanent record keeping. HIGHDEF® DAB Chromogen/Substrate Set is more sensitive and stable than traditional working DAB solutions.
 
Technical Info/Product Notes:Interpretation of the results will be the sole responsibility of the user.
 

Related Products

HIGHDEF® Hematoxylin 

Ready-to-use histologic stain with enhancers
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC106-0100 100 ml Inquire for pricing
Do you need bulk/larger quantities?
 

IHC/ISH Peroxidase Block 

IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC107-0100 100 ml Inquire for pricing
Do you need bulk/larger quantities?
 

POLYVIEW® PLUS AP (anti-rabbit) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC110-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

SAVIEW® PLUS AP Reagent 

High sensitivity, low background, streptavidin-based nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC111-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

SAVIEW® PLUS HRP reagent 

High sensitivity, low background streptavidin-based nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC102-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

POLYVIEW® PLUS HRP (anti-rabbit) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC103-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

POLYVIEW® PLUS HRP (anti-mouse) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC104-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® yellow IHC chromogen (HRP) 

Unique high definition yellow IHC chromogen for HRP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-170-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® Red IHC Chromogen (HRP) 

IHC | Print as PDF
 
ADI-950-210-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® blue IHC chromogen (HRP) 

Unique high definition blue IHC chromogen for HRP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-151-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® black IHC chromogen (HRP) 

Unique high definition black IHC chromogen for HRP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-171-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 

MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit 

Superior multiplex IHC kit for efficient detection of multiple antigens in a single tissue sample.
IHC | Print as PDF
 
ADI-950-100-0001 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Immunohistochemistry
Immunohistochemistry
Download as PDF

Brochures
Neuroscience Antibodies for IHC
Neuroscience Antibodies for IHC
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,