The HIGHDEF® DAB Chromogen/Substrate Set can be used in conjunction with peroxidase-based immunostaining systems. Peroxidase from the antibody detection system reacts with hydrogen peroxide substrate to degrade it, which then reacts with DAB, precipitating it at positive sites and yielding a dark brown color.
Cancerous tonsil tissue was incubated with an anti-Ki-67 primary antibody and then stained with POLYVIEW® PLUS HRP (anti-Rabbit) Reagent (Cat. No. ENZ-ACC104) and then color was developed using HIGHDEF® DAB Chromogen/Substrate Set.
Cancerous tonsil tissue was incubated with an anti-Ki-67 primary antibody and then stained with POLYVIEW® PLUS HRP (anti-Rabbit) Reagent (Cat. No. ENZ-ACC104) and then color was developed using HIGHDEF® DAB Chromogen/Substrate Set.
Dual IHC staining performed on formalin-fixed paraffin-embedded tissue section from transgenic mouse brain expressing human mutated APP and PS1. The tissue sections were stained for alpha-amyloid with POLYVIEW® PLUS AP (anti-rabbit) reagent (Prod. No. ENZ-ACC110) and HIGHDEF® Green AP Chromogen/Substrate (Prod. No. ENZ-ACC130), and phospho-tau with POLYVIEW® PLUS HRP (anti-mouse) reagent (Prod. No. ENZ-ACC104) and HIGHDEF DAB Chromogen/Substrate Set (Prod. No. ENZ-ACC105). Picture courtesy of Dr. Karelle Leroy Laboratoire d’histologie, de neuroanatomie et de neuropathologie, Université Libre de Bruxelles, Bruxelles, Belgium.
Dual IHC staining performed on formalin-fixed paraffin-embedded tissue section from human brain with Alzheimer’s disease. The tissue sections were stained for alpha-amyloid with POLYVIEW® PLUS AP (anti-rabbit) reagent (Prod. No. ENZ-ACC110) and HIGHDEF® Green AP Chromogen/Substrate (Prod. No. ENZ-ACC130), and phospho-tau with POLYVIEW® PLUS HRP (anti-mouse) reagent (Prod. No. ENZ-ACC104) and HIGHDEF DAB Chromogen/Substrate Set (Prod. No. ENZ-ACC105). Picture courtesy of Dr. Karelle Leroy Laboratoire d’histologie, de neuroanatomie et de neuropathologie, Université Libre de Bruxelles, Bruxelles, Belgium.
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Product Details
Applications:
IHC ISH (in situ hybridization)
Contents:
HIGHDEF® DAB Chromogen, HIGHDEF® DAB Substrate Buffer
Quantity:
Materials provided for 1,000 tests
Shipping:
Blue Ice Not Frozen
Long Term Storage:
+4°C
Scientific Background:
3,3'-Diaminobenzidine (DAB), a widely used chromogen for immunoperoxidase staining, is well accepted among pathologists because of its increased sensitivity and decreased background when compared to amino ethyl carbazole (AEC). Specimens stained with DAB can be dehydrated, cleared, and mounted for permanent record keeping. HIGHDEF® DAB Chromogen/Substrate Set is more sensitive and stable than traditional working DAB solutions.
Technical Info/Product Notes:
Interpretation of the results will be the sole responsibility of the user.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Are birds pseudoteeth and denticulations related to touch papilla? An investigation in parrot, goose, and chicken: S. Louryan, et al.; Morphologie 107, 238 (2023), Abstract;
The novel TAK1 inhibitor handelin inhibits NF-κB and AP-1 activity to alleviate elastase-induced emphysema in mice: H.J. Yun; Life Sci. 319, 121388 (2023), Abstract;
A novel C-terminal heat shock protein 90 inhibitor that overcomes STAT3-Wnt-β-catenin signaling-mediated drug resistance and adverse effects: H.J. Lee, et al.; Theranostics 12, 105 (2022), Abstract;
Novel Allosteric Inhibitors of Deoxyhypusine Synthase against Malignant Melanoma: Design, Synthesis, and Biological Evaluation: K.L. Liu, et al.; J. Med. Chem. 1021, 582 (2021), Abstract;
Role of Inflammation and Redox Status on Doxorubicin-Induced Cardiotoxicity in Infant and Adult CD-1 Male Mice: A. Reis-Mendes, et al.; Biomolecules 11, 1725 (2021), Abstract;
Inhibition of tryptophan-dioxygenase activity increases the antitumor efficacy of immune checkpoint inhibitors: F. Schramme, et al.; Cancer Immunol. Res. 8, 32 (2020), Abstract;
Tryptophan 2, 3-dioxygenase expression identified in human hepatocellular carcinoma cells and in intratumoral pericytes of most cancers: D. Hoffmann, et al.; Cancer Immunol. Lett. 8, 19 (2020), Abstract;
Tryptophan 2, 3-dioxygenase expression identified in murine decidual stromal cells is not essential for feto-maternal tolerance: D. Hoffmann, et al.; Front. Immunol. 11, 601759 (2020), Abstract; Full Text
Development of a 4-aminopyrazolo[3,4-d]pyrimidine-based dual IGF1R/Src inhibitor as a novel anticancer agent with minimal toxicity: H.J. Lee, et al.; Mol. Cancer 17, 50 (2018), Abstract; Full Text
Hox-A2 protein expression in mouse embryo middle ear ossicles: S. Louryan, et al.; Morphologie 102, 243 (2018), Abstract;
