Immunohistochemistry (1:50-1:200)
Optimal conditions must be determined individually for each application.
Application Notes:
The IDH1 antibody can be used to qualitatively identify the presence of IDH1 R132H protein mutation in FFPE tissue sections using IHC test methods.
For IHC applications: Dilute the concentrate as appropriate in antibody diluent, and then apply 150 µL to the slide, as indicated in the user's IHC protocol.
Purity Detail:
Affinity-purified.
Formulation:
Liquid. In Tris buffer, pH 7.5, containing 1% BSA and <0.1% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Short Term Storage:
+4°C
Long Term Storage:
+4°C
Scientific Background:
IDH1 (Isocitrate Dehydrogenase 1) is an enzyme found in the cytoplasm and perixosomes. IDH1 is a metabolic gene that is often mutated in human cancer. It is involved in metabolic and cellular pathways, specifically the Krebs cycle. Mutations are frequently found in diffuse low-grade gliomas and secondary glioblastomas, but rarely in primary glioblastomas. The most notable mutation in this enzyme is replacing amino acid arginine with histidine at codon 132 (R132H).
Regulatory Status:
RUO - Research Use Only
Formalin-fixed paraffin-embedded glioblastoma tissue stained using Enzo’s IDH1 antibody (Prod. No. ENZ-ABS774). IHC staining was performed on an automated Leica BOND III.