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Aldolase C monoclonal antibody (AT2E11)

 
ENZ-ABS761-0100 100 µl 390.00 USD
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Product Specification

Alternative Name:ALDC, Fructose bisphosphate aldolase C, Brain-type aldolase
 
Clone:AT2E11
 
Host:Mouse
 
Isotype:IgG2aκ
 
Immunogen:Recombinant human Aldolase C (aa 1-364) purified from E. coli.
 
UniProt ID:P09972
 
Species reactivity:Human
 
Applications:ELISA, ICC, IF, WB
 
Purity Detail:Protein A affinity purified
 
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Aldolase C is a member of the class I fructose-biphosphate aldolase family. This protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1, 6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehydes respectively. It is expressed specifically in the hippocampus and Purkinje cells of the brain.
 
Aldolase C monoclonal antibody (AT2E11) SDS-PAGE
The cell line and tissue lysates (40µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with the ALDOC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: PC3 cell lysate, Lane 2: U87MG cell lysate, Lane 3: WiDr cell lysate, Lane 4: Jurkat cell lysate, Lane 5: Mouse brain tissue lysate.
Aldolase C monoclonal antibody (AT2E11) IF U87MG
IF analysis of ALDOC in U87MG cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Aldolase C monoclonal antibody (AT2E11) IF Jurkat
IF analysis of ALDOC in Jurkat cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
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Aldolase C monoclonal antibody (AT2E11) SDS-PAGE Aldolase C monoclonal antibody (AT2E11) IF U87MG Aldolase C monoclonal antibody (AT2E11) IF Jurkat

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