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PP2C α monoclonal antibody (P6C7)

 
ENZ-ABS739-0100 100 µl 390.00 USD
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Product Specification

Alternative Name:Protein phosphatase 1A (formerly 2C) magnesium-dependent α isoform, PDP1
 
Clone:p6C7
 
Host:Mouse
 
Isotype:IgG2bκ
 
Immunogen:Recombinant human PP2Cα/PPM1A (aa 1-382) purified from E. coli.
 
UniProt ID:P35813
 
Source:Purified from hybridoma tissue culture supernatant.
 
Species reactivity:Human
 
Applications:ELISA, ICC, IF, WB
 
Recommended Dilutions/Conditions:Western Blot (1:500-1:2,000).
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified
 
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Protein phosphatase 2C (PP2Calpha) is a Mn2+ - or Mg2+-dependent protein serine/threonine phosphatase that inhibits the human stress-responsive p38 and JNK MAPK pathways and regulates cellular stress response in eukaryotes. The PPM (metal-dependent protein phosphatase) family of Ser/Thr protein phosphatases have recently been shown to down-regulate the stress response pathways in eukaryotes. Within the stress pathway, key signaling_x000D_ kinases, which are activated by protein phosphorylation, have been proposed as the in vivo substrates of PP2Cα, the prototypical member of the PPM family.
 
PP2C alpha monoclonal antibody (P6C7) ICC IF
ICC/IF analysis of PP2C alpha/PPM1A in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
PP2C alpha monoclonal antibody (P6C7) WB
The cell lysates (40 µg) and tissue lysates (40 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha/PPM1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : Jurkat cell lysate; Lane 2 : HeLa cell lysate; Lane 3 : K-562 cell lysate; Lane 4 : MCF7 cell lysate; Lane 5 : A549 cell lysate; Lane 6 : Raji cell lysate; Lane 7 : Mouse kidney tissue lysate; Lane 8 : Mouse brain tissue lysate; Lane 9 : Mouse liver tissue lysate
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PP2C alpha monoclonal antibody (P6C7) ICC IF PP2C alpha monoclonal antibody (P6C7) WB

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