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CD46 monoclonal antibody (AT2G9)

 
ENZ-ABS722-0100 100 µl 355.00 USD
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Product Specification

Alternative Name:Membrane cofactor protein isoform 14, TLX, Trophoblast leukocyte common antigen
 
Clone:AT2G9
 
Host:Mouse
 
Isotype:IgG2aκ
 
Immunogen:Recombinant human CD46 (aa 35-313) purified from E. coli.
 
UniProt ID:P15529
 
Source:Purified from ascites.
 
Species reactivity:Human
 
Applications:ELISA, Flow Cytometry, ICC, IF, IHC, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000).
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified
 
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Membrane Cofactor Protein (CD46) is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 is implicated in the development and/or progression of selected cancer types.
 
CD46 monoclonal antibody (AT2G9) FACS
Flow cytometry analysis of CD46 in PBMC cells. The cell was stained with ENZ-ABS722 at 2-5µg for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
CD46 monoclonal antibody (AT2G9) IF
ICC/IF analysis of CD46 in HeLa cells. The cell was stained with ENZ-ABS722 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
CD46 monoclonal antibody (AT2G9) IHC
Paraffin embedded sections of human colorectal cancer tissue were incubated with anti-human CD46 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
CD46 monoclonal antibody (AT2G9) WB
The cell lysates of Ramos, HeLa and A549 (40µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD46 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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CD46 monoclonal antibody (AT2G9) FACS CD46 monoclonal antibody (AT2G9) IF CD46 monoclonal antibody (AT2G9) IHC CD46 monoclonal antibody (AT2G9) WB

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