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CD73 monoclonal antibody (AT89E9)

 
ENZ-ABS719-0100 100 µl 355.00 USD
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Product Specification

Alternative Name:Ecto-5'-nucleotidase, 5'-NT
 
Clone:AT89E9
 
Host:Mouse
 
Isotype:IgG2bκ
 
Immunogen:Recombinant human CD73 (aa 27-252) purified from E. coli.
 
UniProt ID:P21589
 
Source:Purified from ascites.
 
Species reactivity:Human
 
Applications:ELISA, Flow Cytometry, ICC, IF, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000).
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G affinity purified
 
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates enabling the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.
 
CD73 monoclonal antibody (AT89E9) FACS
Flow cytometry analysis of CD73 in A431 cells. The cell was stained with ENZ-ABS719 at 2-5µg for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
CD73 monoclonal antibody (AT89E9) IF
ICC/IF analysis of CD73 in HeLa cells. The cell was stained with ENZ-ABS719 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
CD73 monoclonal antibody (AT89E9) WB
The cell lysates (40µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD73 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HepG2 cell lysate. Lane 2: WiDr cell lysate. Lane 3: A431 cell lysate.
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CD73 monoclonal antibody (AT89E9) FACS CD73 monoclonal antibody (AT89E9) IF CD73 monoclonal antibody (AT89E9) WB

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