Purified from hybridoma tissue culture supernatant.
Species reactivity:
E. coli
Applications:
IP, WB
Recommended Dilutions/Conditions:
Western Blot (1:1000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~60kDa by Western blot.
Purity Detail:
Protein G affinity purified.
Formulation:
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
GroEL and GroES are E. coli chaperonins, which are homologs of mitochondrial chaperonins Hsp60 and Hsp10. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding with an ATP-dependent mechanism.
Regulatory Status:
RUO - Research Use Only
Western Blot analysis of ENZ-ABS714: Lane 1: MW Marker, Lane 2: GroEL (E. coli), (recombinant) (Prod. No. ADI-SPP-610), Lane 3: E. coli lysate.