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Zap-70 monoclonal antibody (11H40)

 
ENZ-ABS709-0100 100 µg 365.00 USD
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Product Specification

Alternative Name:ζ-chain associated protein kinase
 
Clone:11H40
 
Host:Mouse
 
Isotype:IgG1κ
 
Immunogen:Recombinant full length human Zap-70.
 
UniProt ID:P43403
 
Source:Purified from hybridoma tissue culture supernatant.
 
Species reactivity:Human, Rat
 
Applications:IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~70kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Zeta-associated protein 70 (Zap-70) is a cytoplasmic protein tyrosine required for T cell antigen receptor (TCR) signaling and development. ZAP-70 interacts with the TCR by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex.
 
ENZ-ABS709 IHC A
Immunohistochemistry analysis of human tonsil tissue using Zap-70 mAb (11H40), (Prod. No. ENZ-ABS709), at a dilution of 1:50. Panel A: Isotype Control, Panel B and C: ENZ-ABS709, diluted to 20µg/mL.
ENZ-ABS709 IHC B
Immunohistochemistry analysis of human tonsil tissue using Zap-70 mAb (11H40), (Prod. No. ENZ-ABS709), at a dilution of 1:50. Panel A: Isotype Control, Panel B and C: ENZ-ABS709, diluted to 20µg/mL.
ENZ-ABS709 IHC C
Immunohistochemistry analysis of human tonsil tissue using Zap-70 mAb (11H40), (Prod. No. ENZ-ABS709), at a dilution of 1:50. Panel A: Isotype Control, Panel B and C: ENZ-ABS709, diluted to 20µg/mL.
ENZ-ABS709 WB
Western Blot analysis of Zap-70 mAb (11H40) (Prod. No. ENZ-ABS709): Lane 1: MW Marker, Lane 2: Jurkat lysate, Lane 3: Rat Thymus lysate.
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ENZ-ABS709 IHC A ENZ-ABS709 IHC B ENZ-ABS709 IHC C ENZ-ABS709 WB

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