Product Specification
| Clone: | CB5.4 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant mouse perforin (aa 98-534). |
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| UniProt ID: | P10820 |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Mouse
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| Specificity: | Recognizes an epitope in the region aa 402-534. |
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| Applications: | ICC, IHC (FS), IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects bands of ~66kDa and higher (depending on PTM) by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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Western Blot analysis of Perforin (mouse) mAb (CB5.4) (Prod. No. ENZ-ABS708): Lane 1: MW Marker, Lane 2: CTLL-2 cell lysate, Lane 3: 3T3 cell lysate. Western Blot analysis of Perforin 1 expression in CTLL-2 cell lysate and absence of perforin in the mouse fibroblast cell line 3T3.
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For Research Use Only. Not for use in diagnostic procedures.
