| Alternative Name: | T-cell immunoreceptor with Ig and ITIM domains |
| |
| Clone: | 4A12 |
| |
| Host: | Mouse |
| |
| Isotype: | IgG1 |
| |
| Immunogen: | Extracellular domain of human TIGIT. |
| |
| UniProt ID: | Q495A1 |
| |
| Species reactivity: | Human
|
| |
| Applications: | Flow Cytometry, ICC, IF, IHC (PS), WB
|
| |
| Purity Detail: | Protein A affinity purified. |
| |
| Formulation: | Liquid. In PBS, with 0.02% sodium azide and 50% glycerol. |
| |
| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 µg/ml.
Western blot analysis of TIGIT in over expressing HEK293 cells using ENZ-ABS698 antibody at (A) 0.25 µg/ml , (B) 0.5 ug/ml, and (C) 1 µg/ml.
Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 µg/ml.
Green: TIGIT Antibody [4A12]
Blue: DAPI staining
Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 µg/ml.
Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 µg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.
