| Alternative Name: | Cytotoxic T-lymphocyte associated protein 4, CD152 |
| |
| Clone: | 1E6 |
| |
| Host: | Mouse |
| |
| Isotype: | IgG1 |
| |
| Immunogen: | Extracellular domain of human CTLA-4. |
| |
| UniProt ID: | P16410 |
| |
| Species reactivity: | Human
|
| |
| Applications: | ICC, IF, IHC (PS), WB
|
| |
| Purity Detail: | Protein A affinity purified. |
| |
| Formulation: | Liquid. In PBS containing 0.02% sodium azide and 50% glycerol. |
| |
| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells. CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer. Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Western blot analysis of CTLA-4 in overexpressing HEK293 cells. CTLA-4 antibody at 0.125 and 0.25 µg/mL.
Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 µg/mL.
Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 µg/mL. Red: CTLA4 Antibody [1E6] (RF16011) Blue: DAPI staining
Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 ug/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 ug/mL.
