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DnaK (E. coli) monoclonal antibody (8E2/2)

 
ENZ-ABS680-0200 200 µg 528.00 USD
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Product Specification

Alternative Name:Hsp70
 
Clone:8E2/2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:E. coli DnaK.
 
UniProt ID:P0A6Y8 (strain K12)
 
Source:Purified from hybridoma tissue culture supernatant.
 
Species reactivity:E. coli
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:10,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~70kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.
 

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