Purified from hybridoma tissue culture supernatant.
Species reactivity:
E. coli
Applications:
WB
Recommended Dilutions/Conditions:
Western Blot (1:10,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~70kDa by Western blot.
Purity Detail:
Protein G affinity purified.
Formulation:
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.
Regulatory Status:
RUO - Research Use Only
Western Blot analysis of ENZ-ABS680: Lane 1: MW Marker, Lane 2: DnaK (E. coli) recombinant protein (Prod. No. ADI-SPP-630), Lane 3: E. coli cell lysate.
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Product Literature References
Genetic Programming by Nitric Oxide-Sensing Gene Switch System in Tumor-Targeting Bacteria: Y. Qin, et al.; Biosensors 13, 266 (2023), Abstract;
Omptin Proteases of Enterobacterales Show Conserved Regulation by the PhoPQ Two-Component System but Exhibit Divergent Protection from Antimicrobial Host Peptides and Complement: Y.H. Cho, et al.; Infect. Immun. 91, e00518 (2023), Abstract;
Transcriptional insight into the effect of benzalkonium chloride on resistance and virulence potential in Salmonella Typhimurium: S. Park & H. Yoon; Microbiol. Res. 266, 127240 (2023), Abstract;
OmpR and Prc contribute to switch the Salmonella morphogenetic program in response to phagosome cues: D.L. Escarpa, et al.; Mol. Microbiol. 118, 477 (2022), Abstract;
The expression of aminoglycoside resistance genes in integron cassettes is not controlled by riboswitches: A. Hipólito, et al.; Nucleic Acids Res. 50, 8566 (2022), Abstract;
