Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot.
Purity Detail:
Protein G affinity purified.
Formulation:
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.
Regulatory Status:
RUO - Research Use Only
Western Blot analysis of ENZ-ABS679: Lane 1: MW Marker, Lane 2: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 3: Mouse Liver Microsomes (Prod. No. ADI-LYT-MM100), Lane 4: Rat Liver Microsomes (ADI-LYT-RM100), Lane 5: HeLa (heat shocked), (cell lysate) (Prod. No. ADI-LYC-HL101).
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Product Literature References
CHIP Haploinsufficiency Exacerbates Hepatic Steatosis via Enhanced TXNIP Expression and Endoplasmic Reticulum Stress Responses: J.H. Han, et al.; Antioxidants 12, 458 (2023), Abstract;
Comparative proteomics reveals elevated CCN2 in NGLY1-deficient cells: R. Hetz, et al.; Biochem. Biophys. Res. Commun. 631, 165 (2022), Abstract;
α-Gal A missense variants associated with Fabry disease can lead to ER stress and induction of the unfolded protein response: F. Consolato, et al.; Mol. Genet. Metab. Rep. 33, 100926 (2022), Abstract;