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KDEL monoclonal antibody (10C3)

 
ENZ-ABS679-0050 50 µg 321.00 USD
 
ENZ-ABS679-0200 200 µg 707.00 USD
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Product Details

Alternative Name:Lys-Asp-Glu-Leu, Lysine-aspartic acid-glutamate-leucine
 
Clone:10C3
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
 
UniProt ID:P06761
 
GenBank ID:M14050
 
Source:Purified from hybridoma tissue culture supernatant.
 
Species reactivity:Avian, Insect, Mammalian, Plant, Yeast
 
Specificity:Recognizes proteins containing the KDEL sequence.
 
Applications:ELISA, Flow Cytometry, ICC, IF, IHC (PS), IP, WB
Electron microscopy
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.
 
Regulatory Status:RUO - Research Use Only
 
KDEL monoclonal antibody (10C3) WB
Western Blot analysis of ENZ-ABS679: Lane 1: MW Marker, Lane 2: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 3: Mouse Liver Microsomes (Prod. No. ADI-LYT-MM100), Lane 4: Rat Liver Microsomes (ADI-LYT-RM100), Lane 5: HeLa (heat shocked), (cell lysate) (Prod. No. ADI-LYC-HL101).
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KDEL monoclonal antibody (10C3) WB

Product Literature References

CHIP Haploinsufficiency Exacerbates Hepatic Steatosis via Enhanced TXNIP Expression and Endoplasmic Reticulum Stress Responses: J.H. Han, et al.; Antioxidants 12, 458 (2023), Abstract;
Comparative proteomics reveals elevated CCN2 in NGLY1-deficient cells: R. Hetz, et al.; Biochem. Biophys. Res. Commun. 631, 165 (2022), Abstract;
α-Gal A missense variants associated with Fabry disease can lead to ER stress and induction of the unfolded protein response: F. Consolato, et al.; Mol. Genet. Metab. Rep. 33, 100926 (2022), Abstract;

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