Product Details
Clone: | SAP7F407 |
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Host: | Mouse |
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Isotype: | IgG2aκ |
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Immunogen: | Recombinant rat Bassoon. |
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UniProt ID: | O88778 |
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Gene/Protein Identifier: | 29999 (UniGene) |
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Source: | Purified from hybridoma tissue culture supernatant. |
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Species reactivity: | Human, Mouse, Rat Bovine, Dog
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Applications: | ICC, IF, IHC, IP, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (1:400)
Immunoprecipitation (2.5µg)
Western Blot (1:500 - 1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Bassoon is a 420 kDa protein that localizes at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release. |
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Regulatory Status: | RUO - Research Use Only |
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Western Blot analysis of ENZ-ABS666: Lane 1: NovexTMHiMarkTM MW Marker, Lane 2: Mouse Brain lysate (Prod. No. ADI-LYT-MB100), Lane 3: Rat Brain lysate (Prod. No. ADI-LYT-RB100).
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded rat brain tissue stained with Bassoon monoclonal antibody (SAP7F407), (Prod. No. ENZ-ABS666) at 10µg/ml.
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Product Literature References
Filamin A organizes γ‑aminobutyric acid type B receptors at the plasma membrane: M.L. Jobin, et al.; Nat. Commun.
14, 34 (2023),
Abstract;
Immunohistochemical distribution of proteins involved in glutamate release in subepithelial sensory nerve endings of rat epiglottis: Y. Yamamoto, et al.; Histochem. Cell Biol.
157, 51 (2022),
Abstract;
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