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HER2/Neu monoclonal antibody

Monoclonal antibody to HER2 for use with IHC applications
 
ENZ-ABS381-1000 1 ml Inquire for pricing
 
ENZ-ABS381-0100 100 µl Inquire for pricing
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  • Strong, clear staining with minimal background
  • Cost-effective option compared to competitor's antibody

Product Specification

Alternative Name:Proto-oncogene c-ErbB-2, CD340
 
Host:Mouse
 
Isotype:IgG
 
Immunogen:Recombinant HER2.
 
UniProt ID:P04626
 
Species reactivity:Human
 
Applications:IHC
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:100-1:200)
Optimal conditions must be determined individually for each application.
 
Application Notes:Validated for use with formalin-fixed paraffin embedded (FFPE) samples.
For IHC applications: Dilute the concentrate as appropriate in Antibody Blocking Buffer (ENZ-ACC108), and then apply 150 µL to the slide, as indicated in the user's IHC protocol.
 
Purity Detail:Affinity-purified.
 
Formulation:Liquid. In Tris buffer, pH 7.5, containing 1% BSA and <0.1% sodium azide.
 
Use/Stability:When stored correctly, the antibody is stable until the date indicated on the label. To ensure proper stability of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Scientific Background:The HER2/Neu (c-erbB-2) proto-oncogene is a trans-membrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the HER2/Neu protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2/Neu and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
 
Protocol:Manual Use
  1. Antigen Retrieval: Perform antigen retrieval with Antigen Retrieval Reagent, pH 9 (ENZ-ACC113), for 10 to 30 minutes at 100°C.
  2. Peroxidase Block: Block using IHC/ISH Peroxidase Block (ENZ-ACC107) for five minutes at room temperature.
  3. Primary Antibody: Dilute antibody 1:100-1:200 before applying 150 µL directly to the tissue. Incubate for 10 to 30 minutes at room temperature.
  4. Secondary Antibody: Incubate using POLYVIEW® PLUS (anti-Mouse) HRP Reagent (ENZ-ACC104) or POLYVIEW® PLUS (anti-Mouse) AP Reagent (ENZ-ACC114) for 20 to 30 minutes.
  5. Substrate Development: Incubate using HIGHDEF® DAB Substrate/Chromogen (ENZ-ACC105) for 5 to 10 minutes at room temperature.
  6. Counterstain: Counterstain with hematoxylin for 30 seconds to 5 minutes. Rinse with distilled water.

Automated Staining
For automated staining, we recommend incubating for 30 minutes with the primary antibody and using Antigen Retrieval at pH 9 for 30 minutes.
 
ENZ-ABS381-1
HER2+ breast cancer tissue incubated with HER2/Neu monoclonal antibody (ENZ-ABS381) and then stained with POLYVIEW® PLUS (anti-Mouse) HRP-DAB Kit (ENZ-KIT160).
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ENZ-ABS381-1

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IHC/ISH Peroxidase Block 

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