| ENZ-ABS381-0100 | 100 µl | 448.00 USD |  |
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| ENZ-ABS381-1000 | 1 ml | 2,533.00 USD | |
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| Alternative Name: | Proto-oncogene c-ErbB-2, CD340 |
| Host: | Mouse |
| Isotype: | IgG |
| Immunogen: | Recombinant HER2. |
| UniProt ID: | P04626 |
| Species reactivity: | Human |
| Applications: | IHC |
| Recommended Dilutions/Conditions: | Immunohistochemistry (1:100-1:200) Optimal conditions must be determined individually for each application. |
| Application Notes: | Validated for use with formalin-fixed paraffin embedded (FFPE) samples. For IHC applications: Dilute the concentrate as appropriate in Antibody Blocking Buffer (ENZ-ACC108), and then apply 150 µL to the slide, as indicated in the user's IHC protocol. |
| Purity Detail: | Affinity-purified. |
| Formulation: | Liquid. In Tris buffer, pH 7.5, containing 1% BSA and <0.1% sodium azide. |
| Use/Stability: | When stored correctly, the antibody is stable until the date indicated on the label. To ensure proper stability of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position. |
| Handling: | Avoid freeze/thaw cycles. |
| Shipping: | Blue Ice Not Frozen |
| Long Term Storage: | +4°C |
| Scientific Background: | The HER2/Neu (c-erbB-2) proto-oncogene is a trans-membrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the HER2/Neu protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2/Neu and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer. |
| Protocol: | Manual Use
Automated Staining For automated staining, we recommend incubating for 30 minutes with the primary antibody and using Antigen Retrieval at pH 9 for 30 minutes. |
| Regulatory Status: | RUO - Research Use Only |
POLYVIEW® PLUS HRP (anti-mouse) reagent |
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| High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC104-0150 | 150 tests | 183.00 USD | |
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POLYVIEW® PLUS AP (anti-mouse) reagent |
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| High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC114-0150 | 150 tests | 183.00 USD | |
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Antibody Blocker/Diluent |
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| Ready-to-use antibody blocking buffer and diluent, for use with POLYVIEW® PLUS detection reagents | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC108-0100 | 100 ml | 257.00 USD | |
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HIGHDEF® DAB Chromogen/Substrate Set |
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| Brown chromogen for use with HRP immunohistochemistry staining systems | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC105-0200 | 200 ml | 267.00 USD | |
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IHC/ISH Peroxidase Block |
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| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC107-0100 | 100 ml | 448.00 USD | |
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HIGHDEF® Hematoxylin |
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| Ready-to-use histologic stain with enhancers | ||||||
| IHC, ISH (in situ hybridization) | Print as PDF | ||||||
| ENZ-ACC106-0100 | 100 ml | 61.00 USD | |
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| Download as PDF | |
