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Basic Cytokeratin Recombinant monoclonal antibody (KRTH/1576R)

 
ENZ-ABS331-0100 100 µg 440.00 USD
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Product Specification

Clone:KRTH/1576R
 
Host:Rabbit
 
Isotype:IgGκ
 
Immunogen:Recombinant full length human KRT76.
 
UniProt ID:Q01546
 
Species reactivity:Human
 
Specificity:This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8)
 
Applications:Flow Cytometry, IF, IHC (PS), WB
 
Recommended Dilutions/Conditions:Flow Cytometry (0.5-1 µg/106 cells in 0.1 mL)
Immunofluorescence (1-2 µg/mL)
Western blot (0.5-1 µg/mL)
Immunohistochemistry (formalin-fixed paraffin sections) (0.25-0.5 µg/mL for 30 min at RT)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In 1X PBS containing 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Scientific Background:Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
 

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