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EpCAM Recombinant monoclonal antibody (rMOC-31)

 
ENZ-ABS317-0100 100 µg 385.00 USD
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Product Specification

Alternative Name:Epithelial cell adhesion molecule, Epithelial specific antigen, ESA, CD326
 
Clone:rMOC-31
 
Host:Mouse
 
Isotype:lgG1κ
 
Immunogen:Neuraminidase treated GLS-1 human small cell lung carcinoma cells.
 
UniProt ID:P16422
 
Species reactivity:Human
 
Specificity:Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM.
 
Applications:Flow Cytometry, IF, IHC (PS), WB
 
Recommended Dilutions/Conditions:Flow Cytometry (0.5-1 µg/106 cells in 0.1 mL)
Immunofluorescence (1-2 µg/mL)
Western blot (0.5-1 µg/mL)
Immunohistochemistry (formalin-fixed paraffin sections) (0.5-1 µg/mL for 30 min at RT)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In 1X PBS containing 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Scientific Background:Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
 

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