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CD20 Recombinant monoclonal antibody (rIGEL/773)

 
ENZ-ABS316-0100 100 µg 385.00 USD
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Product Specification

Alternative Name:B-lymphocyte antigen CD20
 
Clone:rIGEL/773
 
Host:Mouse
 
Isotype:lgG2aκ
 
Immunogen:Recombinant full length human CD20.
 
UniProt ID:P11836
 
Species reactivity:Human
 
Applications:Flow Cytometry, IF, IHC (PS)
 
Recommended Dilutions/Conditions:Flow Cytometry (0.5-1 µg/106 cells in 0.1 mL)
Immunofluorescence (0.5-1 µg/mL)
Immunohistochemistry (formalin-fixed paraffin sections) (0.5-1 µg/mL) for 30 minutes at RT
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:This mAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In 1X PBS containing 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Scientific Background:CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
 

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