Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

ERGIC-53 monoclonal antibody (OTI1A8)

 
ENZ-ABS300-0100 100 µl 377.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:ER-Golgi intermediate compartment 53kDa protein, LMAN1
 
Clone:OTI1A8
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Full length recombinant human ERGIC-53.
 
UniProt ID:P49257
 
Species reactivity:Human, Rat
Dog, Monkey
 
Applications:Flow Cytometry, IF, IHC, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (1:100)
Immunofluorescence (1:100)
Immunohistochemistry (1:150)
Western Blot (1:200-1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Affinity purified.
 
Formulation:Liquid. In PBS, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The ER-Golgi intermediate compartment (ERGIC) marker ERGIC-53 is a mannose-specific membrane lectin operating as a cargo receptor for the transport of glycoproteins from the ER to the ERGIC. ERGIC-53 can also be used for studying aspects of protein trafficking in the secretory pathway.
 
ENZ-ABS300 WB2
Western blot analysis of extracts (35µg) from 9 different cell lines by using ERGIC-53 monoclonal antibody (OTI1A8).
ENZ-ABS300 IHC6
Immunohistochemistry staining of paraffin-embedded carcinoma of human prostate tissue using ERGIC-53 monoclonal antibody (OTI1A8).
ENZ-ABS300 IHC5
Immunohistochemistry staining of paraffin-embedded human prostate tissue within the normal limits using ERGIC-53 monoclonal antibody (OTI1A8).
ENZ-ABS300 IF
ERGIC-53 monoclonal antibody (OTI1A8) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERGIC-53.
ENZ-ABS300 Flow2
Flow cytometric analysis of Jurkat cells, using ERGIC-53 monoclonal antibody (OTI1A8) (red), compared to a nonspecific negative control antibody (blue).
Please mouse over
ENZ-ABS300 WB2 ENZ-ABS300 IHC6 ENZ-ABS300 IHC5 ENZ-ABS300 IF ENZ-ABS300 Flow2

Product Literature References

Assembly and release of infectious hepatitis C virus involving unusual oganization of the secretory pathway: M. Triyatni, et al.; World J. Hepatol. 8, 796 (2016), Application(s): Western blot, human serum, Abstract; Full Text
Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus: C.M. Dorobantu, et al. ; PLoS One 11, e1005185 (2015), Application(s): Immunofluorescence microscopy, Abstract; Full Text

General Literature References

Coronavirus envelope (E) protein remains at the site of assembly: P. Venkatagopalan, et al.; Virology 478C, 75 (2015), Abstract;
The golgin GMAP-210 is required for efficient membrane trafficking in the early secretory pathway: P. Roboti, et al.; J. Cell. Sci. 128, 1595 (2015), Abstract; Full Text
Intracellular phospholipase A1gamma (iPLA1gamma) is a novel factor involved in coat protein complex I- and Rab6-independent retrograde transport between the endoplasmic reticulum and the Golgi complex: R.K. Morikawa, et al.; J. Biol. Chem. 284, 26620 (2009), Abstract;
Deletion of 3 residues from the C-terminus of MCFD2 affects binding to ERGIC-53 and causes combined factor V and factor VIII deficiency: B. Nyfeler, et al.; Blood 111, 1299 (2008), Abstract;
The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi: S. Mitrovic, et al.; Mol. Biol. Cell 19, 1976 (2008), Abstract;
Capturing protein interactions in the secretory pathway of living cells: B. Nyfeler, et al.; PNAS 102, 6350 (2005), Abstract; Full Text
pH-induced conversion of the transport lectin ERGIC-53 triggers glycoprotein release: C. Appenzeller-Herzog, et al.; J. Biol. Chem. 279, 12943 (2004), Abstract; Full Text
Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004), Abstract; Full Text
ERGIC-53 and traffic in the secretory pathway: H.P. Hauri, et al.; J. Cell Sci. 113, 587 (2000), Abstract; Full Text
The recycling of ERGIC-53 in the early secretory pathway. ERGIC-53 carries a cytosolic endoplasmic reticulum-exit determinant interacting with COPII: F. Kappeler, et al.; J. Biol. Chem. 272, 31801 (1997), Abstract; Full Text
ERGIC-53 is a functional mannose-selective and calcium-dependent human homologue of leguminous lectins: C. Itin, et al.; Mol. Biol. Cell 7, 483 (1996), Abstract; Full Text
Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway: C. Itin, et al.; J. Cell Biol. 131, 57 (1995), Abstract; Full Text
A dual role for COOH-terminal lysine residues in pre-Golgi retention and endocytosis of ERGIC-53: F. Kappeler, et al.; J. Biol. Chem. 269, 6279 (1994), Abstract; Full Text
The isolated ER-Golgi intermediate compartment exhibits properties that are different from ER and cis-Golgi: A. Schweizer, et al.; J. Cell Biol. 113, 45 (1991), Abstract; Full Text
Identification, by a monoclonal antibody, of a 53-kD protein associated with a tubulo-vesicular compartment at the cis-side of the Golgi apparatus: A. Schweizer, et al.; J. Cell Biol. 107, 1643 (1988), Abstract; Full Text

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
ERGIC
By biological activity:
ERGIC Monoclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,