Product Details
| Alternative Name: | ER-Golgi intermediate compartment 53kDa protein, LMAN1 |
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| Clone: | OTI1A8 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Full length recombinant human ERGIC-53. |
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| UniProt ID: | P49257 |
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| Species reactivity: | Human, Rat
Dog, Monkey
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| Applications: | Flow Cytometry, IF, IHC, WB
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| Recommended Dilutions/Conditions: | Flow Cytometry (1:100)
Immunofluorescence (1:100)
Immunohistochemistry (1:150)
Western Blot (1:200-1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The ER-Golgi intermediate compartment (ERGIC) marker ERGIC-53 is a mannose-specific membrane lectin operating as a cargo receptor for the transport of glycoproteins from the ER to the ERGIC. ERGIC-53 can also be used for studying aspects of protein trafficking in the secretory pathway. |
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| Regulatory Status: | RUO - Research Use Only |
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Flow cytometric analysis of Jurkat cells, using ERGIC-53 monoclonal antibody (OTI1A8) (red), compared to a nonspecific negative control antibody (blue).
ERGIC-53 monoclonal antibody (OTI1A8) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERGIC-53.
Immunohistochemistry staining of paraffin-embedded human prostate tissue within the normal limits using ERGIC-53 monoclonal antibody (OTI1A8).
Immunohistochemistry staining of paraffin-embedded carcinoma of human prostate tissue using ERGIC-53 monoclonal antibody (OTI1A8).
Western blot analysis of extracts (35µg) from 9 different cell lines by using ERGIC-53 monoclonal antibody (OTI1A8).
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Product Literature References
African swine fever virus transmembrane protein pEP84R guides core assembly: A. Alejo, et al.; PLoS Pathog.
19, e1011136 (2023),
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C11orf94/Frey is a key regulator for male fertility by controlling Izumo1 complex assembly: W. Contreras, et al.; Sci. Adv.
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Abstract;
Expression of the Heterotrimeric GP2/GP3/GP4 Spike of an Arterivirus in Mammalian Cells: A.K. Matczuk, et al.; Viruses
14, 749 (2022),
Abstract;
The Envelope protein of SARS-CoV-2 increases intra-Golgi pH and forms a cation channel that is regulated by pH: D.C. Garcia, et al.; J. Physiol.
599, 2851 (2021),
Abstract;
The P429L loss of function mutation of the human glycine transporter 2 associated with hyperekplexia: A. Kitzenmaier, et al.; Eur. J. Neurosci.
50, 3906 (2019),
Abstract;
Full Text
Cutis laxa, exocrine pancreatic insufficiency and altered cellular metabolomics as additional symptoms in a new patient with ATP6AP1-CDG: B. Dimitrov, et al.; Mol. Genet. Metab.
123, 364 (2018),
Abstract;
Surf4 (Erv29p) binds amino-terminal tripeptide motifs of soluble cargo proteins with different affinities, enabling prioritization of their exit from the endoplasmic reticulum: Y. Yin, et al.; PLoS Biol.
16, e2005140 (2018),
Abstract;
Full Text
Assembly and release of infectious hepatitis C virus involving unusual oganization of the secretory pathway: M. Triyatni, et al.; World J. Hepatol.
8, 796 (2016),
Application(s): Western blot, human serum,
Abstract;
Full Text
Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus: C.M. Dorobantu, et al. ; PLoS One
11, e1005185 (2015),
Application(s): Immunofluorescence microscopy,
Abstract;
Full Text
General Literature References
Coronavirus envelope (E) protein remains at the site of assembly: P. Venkatagopalan, et al.; Virology
478C, 75 (2015),
Abstract;
The golgin GMAP-210 is required for efficient membrane trafficking in the early secretory pathway: P. Roboti, et al.; J. Cell. Sci.
128, 1595 (2015),
Abstract;
Full Text
Intracellular phospholipase A1gamma (iPLA1gamma) is a novel factor involved in coat protein complex I- and Rab6-independent retrograde transport between the endoplasmic reticulum and the Golgi complex: R.K. Morikawa, et al.; J. Biol. Chem.
284, 26620 (2009),
Abstract;
Deletion of 3 residues from the C-terminus of MCFD2 affects binding to ERGIC-53 and causes combined factor V and factor VIII deficiency: B. Nyfeler, et al.; Blood
111, 1299 (2008),
Abstract;
The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi: S. Mitrovic, et al.; Mol. Biol. Cell
19, 1976 (2008),
Abstract;
Capturing protein interactions in the secretory pathway of living cells: B. Nyfeler, et al.; PNAS
102, 6350 (2005),
Abstract;
Full Text
pH-induced conversion of the transport lectin ERGIC-53 triggers glycoprotein release: C. Appenzeller-Herzog, et al.; J. Biol. Chem.
279, 12943 (2004),
Abstract;
Full Text
Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem.
279, 47242 (2004),
Abstract;
Full Text
ERGIC-53 and traffic in the secretory pathway: H.P. Hauri, et al.; J. Cell Sci.
113, 587 (2000),
Abstract;
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The recycling of ERGIC-53 in the early secretory pathway. ERGIC-53 carries a cytosolic endoplasmic reticulum-exit determinant interacting with COPII: F. Kappeler, et al.; J. Biol. Chem.
272, 31801 (1997),
Abstract;
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ERGIC-53 is a functional mannose-selective and calcium-dependent human homologue of leguminous lectins: C. Itin, et al.; Mol. Biol. Cell
7, 483 (1996),
Abstract;
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Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway: C. Itin, et al.; J. Cell Biol.
131, 57 (1995),
Abstract;
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A dual role for COOH-terminal lysine residues in pre-Golgi retention and endocytosis of ERGIC-53: F. Kappeler, et al.; J. Biol. Chem.
269, 6279 (1994),
Abstract;
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The isolated ER-Golgi intermediate compartment exhibits properties that are different from ER and cis-Golgi: A. Schweizer, et al.; J. Cell Biol.
113, 45 (1991),
Abstract;
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Identification, by a monoclonal antibody, of a 53-kD protein associated with a tubulo-vesicular compartment at the cis-side of the Golgi apparatus: A. Schweizer, et al.; J. Cell Biol.
107, 1643 (1988),
Abstract;
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