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MDA5 polyclonal antibody

ENZ-ABS299-0100 100 µg 332.00 USD
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Product Specification

Alternative Name:Helicard, Melanoma differentiation-associated gene 5, Interferon-induced helicase C domain-containing protein 1, RH116, CADM-140 autoantigen, RIG-I-like receptor 2, RLR-2
Immunogen:Synthetic peptide corresponding to 16 amino acids from near the center of human MDA5.
UniProt ID:Q9BYX4 (human), Q8R5F7 (mouse)
Species reactivity:Human, Mouse
Applications:ELISA, IF, IHC (PS), WB
Recommended Dilutions/Conditions:Immunofluorescence (10µg/ml)
Immunohistochemistry (5µg/ml)
Western Blot (1-4µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Affinity purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored as recommended.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.
Figure 1: Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.
Figure 2:Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 μg/mL.
Figure 3:Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 μg/mL.
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General Literature References

Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002), Abstract;
Overexpression of Helicard, a CARD-Containing Helicase Cleaved during Apoptosis, Accelerates DNA Degradation: M. Kovacsovics, et al.; Curr. Biol. 12, 838 (2002), Abstract;

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