MDA5 polyclonal antibody



ENZ-ABS299-0100	100 µg	425.00 USD
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Product Details

Alternative Name:	Helicard, Melanoma differentiation-associated gene 5, Interferon-induced helicase C domain-containing protein 1, RH116, CADM-140 autoantigen, RIG-I-like receptor 2, RLR-2

Host:	Rabbit

Isotype:	IgG

Immunogen:	Synthetic peptide corresponding to 16 amino acids from near the center of human MDA5.

UniProt ID:	Q9BYX4 (human), Q8R5F7 (mouse)

Species reactivity:	Human, Mouse

Applications:	ELISA, IF, IHC (PS), WB

Recommended Dilutions/Conditions:	Immunofluorescence (10µg/ml) Immunohistochemistry (5µg/ml) Western Blot (1-4µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Purity Detail:	Affinity purified.

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Use/Stability:	Stable for at least 1 year after receipt when stored as recommended.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Scientific Background:	MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.

Regulatory Status:	RUO - Research Use Only

MDA5 polyclonal antibody Immunohistochemistry

Figure 3:Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 μg/mL.

MDA5 polyclonal antibody Immunofluorescence

Figure 2:Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 μg/mL.

Figure 1: Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.

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Product Literature References

Comparison of tissue tropism and host response to enteric and respiratory enteroviruses: I.C. Filipe, et al.; PLoS Pathog. 18, e1010632 (2022), Abstract;

MDA5 disease variant M854K prevents ATP-dependent structural discrimination of viral and cellular RNA: Q. Yu, et al.; Nat. Commun. 12, 6668 (2021), Abstract;

General Literature References

Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002), Abstract;

Overexpression of Helicard, a CARD-Containing Helicase Cleaved during Apoptosis, Accelerates DNA Degradation: M. Kovacsovics, et al.; Curr. Biol. 12, 838 (2002), Abstract;

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