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V5-tag polyclonal antibody

 
ENZ-ABS286-0100 100 µg 230.00 USD
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Product Specification

Host:Rabbit
 
Isotype:IgG
 
Immunogen:Synthetic peptide corresponding to GKPIPNPLLGLDST.
 
Species reactivity:Species independent
 
Applications:WB
 
Purity Detail:Antigen affinity chromatography
 
Formulation:Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the epitope tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered protein, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.
 

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