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GAPDH monoclonal antibody (GA1R) (HRP conjugate)

 
ENZ-ABS276-0050 50 µl 147.00 USD
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Product Specification

Alternative Name:Glyceraldehyde-3-phosphate dehydrogenase
 
Clone:GA1R
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Full-length recombinant GAPDH.
 
UniProt ID:P04406
 
Species reactivity:Human, Mouse, Rat
Chicken, E. coli, Hamster, Insect, Rabbit, Yeast
 
Specificity:Recognizes GAPDH in multiple species, including human, mouse, rat, bacteria, chicken, hamster, insect, rabbit and yeast.
 
Applications:WB
 
Purity Detail:Purified
 
Formulation:Liquid. In PBS containing a proprietary stabilizer and no preservative.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in Western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
 
ENZ-ABS276 WB
Fig. 1. Western Blot with GAPDH, mAb (GA1R) (HRP conjugate) (ENZ-ABS276-0050). Western blot analysis of GAPDH was performed by loading 50ug of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated GAPDH monoclonal antibody (ENZ-ABS276-0050) at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed.
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ENZ-ABS276 WB

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