| Alternative Name: | Carboxyl terminus of Hsc70-interacting protein |
| |
| Host: | Rabbit |
| |
| Isotype: | IgG |
| |
| Immunogen: | Synthetic peptide corresponding to aa 218-232 of human CHIP. |
| |
| UniProt ID: | Q9UNE7 |
| |
| Species reactivity: | Human, Mouse
|
| |
| Applications: | WB
|
| |
| Application Notes: | Detects a band of ~35kDa by Western blot. |
| |
| Purity Detail: | Antigen affinity chromatography |
| |
| Formulation: | Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | CHIP has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency.
CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
