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Cardif/MAVS (human) monoclonal antibody (58N3B6)

 
ENZ-ABS259-0100 100 µg 462.00 USD
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Product Specification

Alternative Name:CARD adapter inducing interferon-β, IPS-1, Interferon-β promoter stimulator protein 1, Mitochondrial antiviral signalling protein, VISA, Virus-induced signalling adapter
 
Clone:58N3B6
 
Host:Mouse
 
Isotype:IgG2bκ
 
Immunogen:Human MAVS (IPS-1)
 
UniProt ID:Q7Z434
 
Gene/Protein Identifier:57506 (Entrez GeneID)
 
Species reactivity:Human
 
Applications:IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:10-1:2,000) (paraffin sections 3-5µg/ml)
Western Blot (2-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G purified.
 
Formulation:Liquid. In 0.2ml PBS containing 0.05% BSA and 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.
 

Product Literature References

Retinoic acid-inducible gene-I-like receptor (RLR)-mediated antiviral innate immune responses in the lower respiratory tract: roles of TRAF3 and TRAF5: Y. Chiba, et al.; Biochem. Biophys. Res. Commun. 467, 191 (2015), Application(s): Immunoblot analyses of human tissue, Abstract;

General Literature References

CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006), Abstract;
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005), Abstract;
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005), Abstract;
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005), Abstract;
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005), Abstract;

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For Research Use Only. Not for use in diagnostic procedures.
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