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Matrix Gla protein monoclonal antibody (52.1#1)

 
ENZ-ABS254-0050 50 µg Inquire for pricing
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Product Specification

Alternative Name:MGP
 
Clone:52.1#1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to aa 3-15 of non-phosphorylated human MGP (matrix Gla protein).
 
UniProt ID:P08493
 
Species reactivity:Human
 
Specificity:Recognizes both carboxylated and non-carboxylated human matrix Gla protein.
 
Crossreactivity:Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset (100%); Dog, Horse, Rabbit (92%); Mouse, Rat, Bovine, Bat, Pig, Opossum (85%).
 
Applications:IF, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunofluorescence (1:50-1:500)
Immunohistochemistry (paraffin sections, 1:50-1:500)
Immunoprecipitation (1-2 μg per 100-500 μg)
Western Blot (1:200-1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In 0.5 ml PBS containing < 0.1% sodium azide and 0.1% gelatin.
 
Use/Stability:Stable for 1 year when stored at +4°C.
 
Handling:Do not freeze.
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
Scientific Background:Matrix Gla protein (MGP) is a potent inhibitor of vascular calcification. MGP is mainly synthesized and expressed in cartilage and the vessel wall, where it is produced by the chondrocytes and vascular smooth muscle cells, respectively. During biosynthesis, MGP undergoes two forms of  post-translational modifications: the phosphorylation of 3 serine residues and the γ-carboxylation of 5 glutamate residues. Phosphorylation of MGP is essential for its regular cellular secretion, and the vitamin K-dependent carboxylation is crucial for the calcification inhibitory activity. MGP plays a role in bone metabolism and atherosclerosis.
 

General Literature References

Post-translational modifications regulate matrix Gla protein function: importance for inhibition of vascular smooth muscle cell calcification: L.J. Schurgers, et al.; J. Thromb. Haemost. 5, 2503 (2007), Abstract; Full Text
Characteristics and performance of an immunosorbent assay for human matrix Gla-protein: L.J. Schurgers, et al.; Clin. Chim. Acta 351, 131 (2005), Abstract;
Novel conformation-specific antibodies against matrix gamma-carboxyglutamic acid (Gla) protein: undercarboxylated matrix Gla protein as marker for vascular calcification: L.J. Schurgers, et al.; Arterioscler. Thromb. Vasc. Biol. 25, 1629 (2005), Abstract; Full Text
Matrix gla protein accumulates at the border of regions of calcification and normal tissue in the media of the arterial vessel wall: H.M. Spronk, et al.; BBRC 289, 485 (2001), Abstract; Full Text
Role of Vitamin K and vitamin K-dependent proteins in vascular calcification: L.J. Schurgers, et al.; Z. Kardiol. 90, 57 (2001), Abstract;
Assay for Human Matrix Gla Protein in Serum: Potential Applications in the Cardiovascular Field: L.A. Braam, et al.; Arterioscler. Thromb. Vasc. Biol. 20, 1257 (2000), Abstract; Full Text
Molecular structure, chromosome assignment, and promoter organization of the human matrix Gla protein gene: L. Cancela, et al.; J. Biol. Chem. 265, 15040 (1990), Abstract; Full Text
The cDNA and derived amino acid sequences for human and bovine matrix Gla protein: M.C. Kiefer, et al.; Nucleic Acids Res. 16, 5213 (1988), Abstract; Full Text

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