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DiSH™ Kit

Reduce labor time and costs using a novel technology for rapid hybridoma production
 
ENZ-71001-0001 1 Kit 1,050.00 USD
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  • Unique technology for more rapid hybridoma production
  • Decreased time and labor costs
  • Improved selection of specific, stable hybridomas
  • Minimal manipulation of cloned cell lines relative to approaches requiring Limiting Dilution Subcloning
A genetically-modified myeloma partner which creates hybridomas that both secrete antigen-specific antibody and also express antibody on the cell surface of the hybridoma.

The DiSH Kit contains Abeome's patented SP2ab myeloma fusion partner PLUS culture, fusion, selection and recovery media for creation and isolation of hybridomas by fluorescent or magnetic separation.

Product Specification

Alternative Name:Hybridoma Kit
 
Application Notes:Uses:
Hybridoma generation and Cloning
Cell line development
Recommended for generation, selection and cloning of mouse monoclonal hybridomas.
 
Quantity:For fusion of 1x108 total lymphocytes
 
Use/Stability:When stored properly, these reagents are stable for one year from date received.
 
Handling:Upon receipt, remove the vial of cell line from the box and store in liquid nitrogen. Store the remaining reagents at -20°C. Avoid repeated freezing and thawing by storing aliquots at -20°C.
 
Shipping:Shipped on Dry Ice
 
Kit/Set Contains:1 vial of Sp2ab myeloma partner cells
1.5 ml PEG
200 ml PCM (Prefusion Medium)
(2X) 200 ml WCM (Wash Medium)
200 ml FCM (Fusion Medium)
90 ml AbeoClone™ Medium
(2X) 200 ml RCM (Recovery Medium)
200 ml ECM (Expansion Medium)
 
Technical Info/Product Notes:Application Note:
Anti-hESC mAb Hesca-2 Binds to a Glycan Epitope Commonly Found on Carcinomas
 

Product Literature References

Anti-Human Embryonic Stem Cell Monoclonal Antibody Hesca-2 Binds to a Glycan Epitope Commonly Found on Carcinomas: M.G. Shoreibah, et al.; Stem Cells Dev. 20, 515 (2011), Abstract; Full Text
Engineered cell surface expression of membrane immunoglobulin as a means to identify monoclonal antibody-secreting hybridomas: P.W. Price, et al.; J. Immunol. Methods 343, 28 (2009), Application(s): Hyridoma screening, Abstract; Full Text

General Literature References

Antibody production by hybridomas: J.W. Goding; J. Immunol. Methods 39, 285 (1980), Abstract;
Detection of specific hybridoma clones by replica immunoadsorption of their secreted antibodies: J. Sharon, et al.; PNAS 76, 1420 (1979), Abstract; Full Text
Continuous culture of fused cells secreting antibody of predefined specificity: G. Köhler & C. Milstein; Nature 256, 495 (1975), Abstract; Full Text

Related Products

PCM (Prefusion Medium) 

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ENZ-70001-0200 200 ml 62.00 USD
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WCM (Wash Medium) 

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ENZ-70002-0200 200 ml 53.00 USD
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FCM (Fusion Medium) 

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ENZ-70003-0200 200 ml 62.00 USD
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AbeoClone™ (HAT Semi-solid Medium) 

Semi-solid HAT media optimized for selection of hybridoma colonies
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ENZ-70004-0090 90 ml 314.00 USD
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RCM (Recovery Medium) 

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ENZ-70005-0200 200 ml 314.00 USD
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ECM (Expansion Medium) 

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ENZ-70006-0200 200 ml 104.00 USD
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PEG (polyethylene glycol) 

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ENZ-70007-0001 1.5 ml 53.00 USD
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Sp2ab myeloma fusion partner 

Myeloma fusion partner for rapid hybridoma production
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ENZ-70008-0001 1 Vial 945.00 USD
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