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AbeoClone™ (HAT Semi-solid Medium)

Semi-solid HAT media optimized for selection of hybridoma colonies
ENZ-70004-0090 90 ml 320.00 USD
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  • Each colony originating from a single hybridoma can be isolated and characterized
  • Avoids the isolation of multiple identical clones
  • Minimizes loss of useful clones due to overgrowth by other faster-growing clones
AbeoClone™ HAT Medium may be used directly after cell fusion to select for and clone hybridomas, or may be used in conjunction with Abeome’s patented DiSH™ (Direct Selection of Hybridomas) Kit to isolate antigen-specific hybridomas after magnetic separation. It is fully compatible with robotic colony picking.
The media itself is semi-solid HAT-containing growth medium optimized for the rapid growth and isolation of clonal hybridomas. It contains a proprietary blend of growth factors and conditioned media optimized for single-cell growth of newly-fused hybridomas.

Product Specification

Alternative Name:Hybridoma Dish™ Kit semi-solid medium
Application Notes:Uses:
Hybridoma generation
Cell line development
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Handling:Refrigerate or freeze upon arrival.

Product Literature References

Anti-Human Embryonic Stem Cell Monoclonal Antibody Hesca-2 Binds to a Glycan Epitope Commonly Found on Carcinomas: M.G. Shoreibah, et al.; Stem Cells Dev. 20, 515 (2011), Abstract; Full Text
Engineered cell surface expression of membrane immunoglobulin as a means to identify monoclonal antibody-secreting hybridomas: P.W. Price, et al.; J. Immunol. Methods 343, 28 (2009), Application(s): Hyridoma screening, Abstract; Full Text

General Literature References

Antibody production by hybridomas: J.W. Goding; J. Immunol. Methods 39, 285 (1980), Abstract;
Detection of specific hybridoma clones by replica immunoadsorption of their secreted antibodies: J. Sharon, et al.; PNAS 76, 1420 (1979), Abstract; Full Text
Continuous culture of fused cells secreting antibody of predefined specificity: G. Köhler & C. Milstein; Nature 256, 495 (1975), Abstract; Full Text

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