Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

LEADING LIGHT® Sclerostin-LRP Interaction Screening System

Novel Cell-free Sclerostin/LRP Binding Assay Ideal for Primary Inhibitor Screens
 
ENZ-61003-0001 96 wells 380.00 USD
Do you need bulk/larger quantities?
 
  • High sensitivity chemiluminescent readout (signal-to-noise ratio of ≥ 10)
  • Rapid protocol yields results in just 2.5 hours
  • No cell lines or transfection reagents required
  • Reproducible, high-throughput amenable assay (Z’-factor 0.871)
  • Amenable for high-throughput screening applications
The LEADING LIGHT® Sclerostin Screening System is a 1x96 well plate assay based on Sclerostin–LRP interaction. This system contains engineered LRP5-alkaline phosphatase (ALP) and human Sclerostin immobilized in a multi-well plate. LRP5-alkaline phosphatase binds to Sclerostin coated on the bottom of the well and is detected by the activity of alkaline phosphatase remaining after a washing step. This system can be used for drug screening to identify small molecule compounds, antibodies, DNA aptamers and peptides capable of modulating sclerostin-LRP interaction.
ENZ-61003 inhibition
Inhibition of Sclerostin binding by Acid Green 25. The LRP5-ALP was bound to sclerostin in the presence of the indicated concentration of Acid Green 25 used to inhibit this interaction.
ENZ-61003 kit outlines
ENZ-61003 box
Please mouse over
ENZ-61003 inhibition ENZ-61003 kit outlines ENZ-61003 box

Product Specification

Alternative Name:Sclerostin-LRP binding kit, SOST
 
Assay Time:2.5 hours
 
Applications:FUNC, Chemiluminescence, HTS
 
Species reactivity:Human
 
Handling:Upon receipt, the kit should be stored at -20° C until kit is used. Upon first use of kit, PBS, Wash Buffer, and ALP Substrate may be stored at 4°C. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:Sclerostin microtiter plate, LRP5-ALP fusion protein concentrate, ALP concentrate (negative control), PBS solution, Blocking agent, wash buffer, acid green 25, ALP substrate reagent, plate sealer
 
Scientific Background:Sclerostin is encoded by the SOST gene and serves as a negative regulator of bone formation. Wnt ligands bind to Frizzled (Fz) and LRP5/6 receptors to trigger the canonical Wnt signaling cascade, which has been reported to play an important role in bone development. Sclerostin can bind to LRP5/6 to inhibit the canonical Wnt signaling. Antibodies against Sclerostin can block Sclerostin-mediated Wnt inhibition in cell based assays and increase bone mineral density in animal models. Importantly clinical trial studies demonstrate that antibodies against Sclerostin can also increase human bone mineral density. Consequently, Sclerostin is thought to be a superior therapeutic target for osteoporosis treatment over current implemented methods such as treatment with bisphosphonate derivatives.
 
Technical Info/Product Notes:Application Notes:
Detecting LEADING LIGHT® Sclerostin-LRP Screening System using the CLARIOstar® and LVF-Monochromator
 
UniProt ID:Q9BQB4
 

Product Literature References

Structural and functional insights into sclerostin-glycosaminoglycan interactions in bone: J. Salbach-Hirsch, et al.; Biomaterials 67, 335 (2015), Abstract;
WNT1-induced secreted protein-1 (WISP1), a novel regulator of bone turnover and Wnt signaling: A. Maeda, et al.; J. Biol. Chem. 290, 14004 (2015), Abstract;

Related Products

LEADING LIGHT® Wnt Reporter Assay Starter Kit 

Chemiluminescent cell-based assay for discovering Wnt pathway modulators
FUNC, Chemiluminescence, HTS | Print as PDF
 
ENZ-61001-0001 96 wells 946.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
Assay Layout Sheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By target:
Sclerostin
By biological activity:
Sclerostin Detection kit
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Integrated solutions for screening Wnt regulators
Integrated solutions for screening Wnt regulators
Download as PDF

Brochures
Stem Cells
Stem Cells
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,