Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Dkk-1 (mouse), (recombinant) (active)

Modulator of embryogenesis and known effector of tissue development and morphogenesis
 
ENZ-60002-C005 5 µg 205.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Dickkopf-related protein-1
 
Sequence:Mouse Dkk-1 amino acids 33-272, as well as a TEV recognition site and 6 histidines at the C terminus.
 
MW:~40kDa (SDS-PAGE)
 
Source:Produced in insect cells. Produced in a baculovirus expression system.
 
UniProt ID:O54908
 
Quantity:5µg
 
Formulation:Lyophilized from a solution containing 1X PBS, 1% CHAPS, and 0.1% BSA.
 
Purity:≥80% (SDS-PAGE)
 
Endotoxin Content:<1.0EU/µg (LAL method)
 
Biological Activity:Determined measuring the ability to inhibit the induction of the LEADING LIGHT® Wnt Reporter Cell Line (Prod. No. ENZ-61002) induced for at least 6 hours with 200 ng/ml Wnt3a (Prod. No. ENZ-60001). The EC50 of this inhibition is 45-55 ng/ml.
 
Reconstitution:Reconstitute by adding 1.5ml water to make a 3.3µg/ml solution.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-80°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 6 months after receipt when stored as supplied at -80°C.
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:Dickkopf-related protein 1 (Dkk-1), which belongs to the dickkopf family, is a secreted protein with two cysteine rich domains and is involved in embryonic development and many human diseases. The regulation of Wnt signaling and Dkk1 inhibition has implications for bone formation and homeostatis. Dkk-1 can inhibit Wnt signaling by binding to its receptors LRP5/6 and kremen1/2 to form a complex, which can prevent Wnt from binding to LRP5/6. The predicted mouse Dkk1 protein is 272 amino acid residues with an N-terminal signal peptide. It shares 86% amino acid identity to human Dkk-1 protein.
 
Technical Info/Product Notes:Application Note: New LEADING LIGHT Wnt Reporter Assay with Highest Sensitivity in Response to Wnt Effectors
 

General Literature References

Function and biological roles of the Dickkopf family of Wnt modulators: C. Niehrs; Oncogene 25, 7469 (2006), Abstract;
Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signalling: B. Mao et al.; Nature 417, 664 (2002), Abstract;
LDL-receptor-related protein 6 is a receptor for Dickkopf proteins: B. Mao et al.; Nature 411, 321 (2001), Abstract;

Related Products

Wnt-3a (mouse), (recombinant) 

Modulator of embryogenesis, cell proliferation, stem cell differentation, and bone, glucose and lipid metabolism
Produced in CHO cells, ≥80% (SDS-PAGE) | Print as PDF
 
ENZ-60001-C001 1 µg 156.00 USD
Do you need bulk/larger quantities?
 

LEADING LIGHT® Wnt Reporter Assay Starter Kit 

Chemiluminescent cell-based assay for discovering Wnt pathway modulators
FUNC, Chemiluminescence, HTS | Print as PDF
 
ENZ-61001-0001 96 wells 946.00 USD
Do you need bulk/larger quantities?
 

LEADING LIGHT® Wnt Cell Line Medium Pack 

For use with the LEADING LIGHT® Wnt Reporter Cell Line
Print as PDF
 
ENZ-60003-0001 1 Pack 342.00 USD
Do you need bulk/larger quantities?
 

LEADING LIGHT® Wnt Cell Line Assay Reagents 

For use with the LEADING LIGHT® Wnt Reporter Cell Line
Print as PDF
 
ENZ-60004-0001 1 Pack 420.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
DKK
By biological activity:
DKK Recombinant protein
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Integrated solutions for screening Wnt regulators
Integrated solutions for screening Wnt regulators
Download as PDF

Brochures
Stem Cells
Stem Cells
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,