| MW: | ~40kDa (SDS-PAGE) |
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| Source: | Cell culture |
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| UniProt ID: | P27467 |
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| Formulation: | Lyophilized solid. |
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| Purity: | >75% by SDS-PAGE and >75% by HPLC analyses |
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| Endotoxin Content: | Less than 0.2 ng per µg (2EU/µg) |
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| Biological Activity: | Wnt-3a can induce β-catenin mediated response in a TCF/LEF reporter gene luciferase assay. |
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| Reconstitution: | Prepare 5 μg/mL stock solution of Wnt3a by adding 200 μL deionized water into the vial of lyophilized Wnt3a. |
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| Shipping: | Dry Ice |
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| Short Term Storage: | -80°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for at least 12 months after receipt when stored, as supplied, at -80°C. Following reconstitution, stable for three months when stored at or below -20°C. |
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| Scientific Background: | Wnt expression and signal transduction have been identified as critical components in regulating embryogenesis, cell proliferation, and bone, glucose and lipid metabolism. Wnt-3a signalling also plays a role in regulating the differentation of stem cells. Wnt-3a is a member of the highly conserved, structurally related cysteine-rich Wnt family glycoproteins important for normal developmental processes and oncogenesis. Wnt-3a proteins are secreted and bind to receptors of the Frizzled and LRP families on the cell surface, which leads to the stabilization of β-catenin and increase of β-catenin translocation into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. The predicted mouse Wnt-3a protein of 352 amino acid residues and contains an N-terminal signal peptide for secretion. It shares 96% amino acid identity to human Wnt-3a protein. Due to glycosylation, it migrates at an apparent molecular weight of ~40 kDa by SDS-PAGE analysis under reducing conditions. |
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| Technical Info/Product Notes: | Application Note:
New LEADING LIGHT Wnt Reporter Assay with Highest Sensitivity in Response to Wnt Effectors |
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| Regulatory Status: | RUO - Research Use Only |
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