Mito-ID™ Red antifade reagent with DAPI

Mitochondria dye



ENZ-53003-M010	10 ml	70.00 USD

Developed specifically for Mito-ID™ Red mitochondrial stain, but works well with many other stains, such as fluorescein and rhodamine. Causes little or no quenching. Use of the Mito-ID™ Red Antifade Reagent with DAPI (Prod. No. ENZ-53003) with the Mito-ID™ Red dye, or other stains, significantly extends the photobleaching half-life of the fluorescent stain. The addition of DAPI to the mounting medium allows for staining of the nucleus.

Product Specification

Application:	Developed specifically for Mito-ID™ Red mitochondrial stain, but works well with many other stains.

Long Term Storage:	-80°C

Handling:	Avoid freeze/thaw cycles. Protect from light.

Miscellaneous/General:	All fluorescent dyes fade (photobleach) when exposed to excitation light. The amount of photobleaching depends on the dye, the intensity and the time of illumination. Many products have been developed to prevent fading of fluorescent dyes. These products often work well for one or more dyes, but often cause problems, such as degradation of other dyes. Mito-ID™ Red Antifade Reagent with DAPI (Prod. No. ENZ-53003) was developed specifically for Mito-ID™ Red mitochondrial stain, but works well with many other stains, such as fluorescein and rhodamine.

Background / Technical Information:	For instructions on use, please refer to the Mito-ID™ Red Detection Kit (Prod. No. ENZ-51007-500) user manual. The Mito-ID™ Red Antifade Reagent with DAPI is recommended for use on fixed cells and tissues that have been stained with the Mito-ID™ Red dye or other fluorescent stain. Cells can be viewed immediately, or if long term storage is desired, the reagent can be allowed to harden overnight. Staining patterns of Mito-ID™ dye are sharper if viewed immediately. The Mito-ID™ Red Antifade Reagent with DAPI is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.

General Literature References

Influence of refractive-index mismatch in high-resolution three-dimensional confocal microscopy.: A. Diaspro et al.; Appl. Opt. 41, 685 (2002), Abstract;

Quantitative comparison of anti-fading mounting media for confocal laser scanning microscopy. : M. Ono et al.; J. Histochem. Cytochem. 49, 305 (2001), Abstract; Full Text