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Pyocyanin

ROS inducer
 
ENZ-53001-C001 1 µmol 60.00 USD
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Cytotoxic pigment secreted by Pseudomonas aeruginosa, which frequently infects the lungs of immuno-suppressed patients, as well as those with cystic fibrosis. Pyocyanin toxicity results from its ability to undergo reduction by NAD(P)H and subsequent generation of superoxide and H2O2 directly in the lungs.

Product Specification

Alternative Name:Sanasin, 1-hydroxy-N-methylphenazine
 
Formula:C13H10N2O
 
MW:210.2
 
CAS:85-66-5
 
Quantity:1 μmol
 
Formulation:Lyophilized.
 
Purity:≥98% (HPLC)
 
Quality Control:Quality assayed by measuring the ability to increase the total ROS and superoxide levels in live cells using flow cytometry assays.  Pyocyanin treatment of HeLa cells has to increase the percentage of green positive cells (total ROS levels) over 35% and orange positive cells (superoxide levels) over 70% compared to uninduced cells.
 
Solubility:Soluble in DMSO or dimethyl formamide.
 
Reconstitution:Reconstitute the contents of the vial in 100μl solvent to yield a 10mM stock solution.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 1 year after receipt when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Pyocyanin is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
enz-53001
enz-53001 fig
Figure: Pyocyanin is reduced by NAD(P)H and generates superoxide and H2O2.
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enz-53001 enz-53001 fig

Product Literature References

Functional pseudogenes inhibit the superoxide production: W. Xu, et al.; Precision Medicine 2, e745 (2015), Application(s): Cell Culture, Fluorescence Microscopy, Full Text
Impairment of apoptotic cell engulfment by pyocyanin, a toxic metabolite of Pseudomonas aeruginosa.: S.M. Bianchi, et al.; Am. J. Respir. Crit. Care Med. 177, 35 (2008), Abstract;
Pyocyanin production by Pseudomonas aeruginosa induces neutrophil apoptosis and impairs neutrophil-mediated host defenses in vivo.: L. Allen, et al.; J. Immunol. 174, 3643 (2005), Abstract;

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